Characterization of a red pigment from Fusarium chlamydosporum exhibiting selective cytotoxicity against human breast cancer MCF-7 cell lines.
J Appl Microbiol
; 125(1): 148-158, 2018 Jul.
Article
en En
| MEDLINE
| ID: mdl-29527765
ABSTRACT
AIM:
This research aims to characterize the pigment produced by isolated fungi and to evaluate its anticancer activities. METHODS ANDRESULTS:
Pigment-producing fungi was isolated and identified as Fusarium chlamydosporum. The pigment was extracted with chloroform, purified by preparative TLC and characterized by Fourier transmittance infrared, electron spray ionization mass spectroscopy, liquid chromatography mass spectroscopy and nuclear magnetic resonance (NMR) (1 HNMR, 13 C NMR) spectral analysis, which revealed the pigment to be 'long chain hydrocarbons with poly unsaturated groups' (m/z 702). Pigment stability varied with different pH, temperature and sunlight conditions. The pigment-induced cell death in human breast adenocarcinoma cells MCF-7 and showed no significant toxicity in CHOK 1 cells. Lipid peroxidation assay revealed that treatment with pigment was able to reduce the lipid peroxidation caused by H2 O2 in MCF-7 cells.CONCLUSIONS:
The F. chlamydosporum pigment is a compound of long-chain hydrocarbons with poly unsaturated groups, possessing selective cytotoxicity in MCF-7 cancer cell lines. SIGNIFICANCE AND IMPACT OF THE STUDY The pigment can be used as a colouring agent in cosmetics. Its anticancer potential can be used in production of therapeutics in increasing demand cancer research.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Pigmentos Biológicos
/
Supervivencia Celular
/
Fusarium
/
Micotoxinas
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Appl Microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
2018
Tipo del documento:
Article
País de afiliación:
India