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Proteomic identification of galectin-11 and 14 ligands from Haemonchus contortus.
Sakthivel, Dhanasekaran; Swan, Jaclyn; Preston, Sarah; Shakif-Azam, M D; Faou, Pierre; Jiao, Yaqing; Downs, Rachael; Rajapaksha, Harinda; Gasser, Robin; Piedrafita, David; Beddoe, Travis.
Afiliación
  • Sakthivel D; Department of Animal, Plant and Soil Science and Centre for AgriBioscience (AgriBio), La Trobe University, Bundoora, Victoria, Australia.
  • Swan J; Department of Biochemistry and Molecular Biology, Monash University, Clayton, Australia.
  • Preston S; School of Applied and Biomedical Sciences, Federation University, Churchill, Australia.
  • Shakif-Azam MD; Department of Animal, Plant and Soil Science and Centre for AgriBioscience (AgriBio), La Trobe University, Bundoora, Victoria, Australia.
  • Faou P; Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Melbourne, Australia.
  • Jiao Y; Faculty of Science and Technology, Federation University, Ballarat, Australia.
  • Downs R; School of Applied and Biomedical Sciences, Federation University, Churchill, Australia.
  • Rajapaksha H; Department of Biochemistry & Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, Australia.
  • Gasser R; Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Melbourne, Australia.
  • Piedrafita D; Department of Biochemistry & Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, Australia.
  • Beddoe T; Department of Biochemistry & Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, Australia.
PeerJ ; 6: e4510, 2018.
Article en En | MEDLINE | ID: mdl-29576976
Haemonchus contortus is the most pathogenic nematode of small ruminants. Infection in sheep and goats results in anaemia that decreases animal productivity and can ultimately cause death. The involvement of ruminant-specific galectin-11 (LGALS-11) and galectin-14 (LGALS-14) has been postulated to play important roles in protective immune responses against parasitic infection; however, their ligands are unknown. In the current study, LGALS-11 and LGALS-14 ligands in H. contortus were identified from larval (L4) and adult parasitic stages extracts using immobilised LGALS-11 and LGALS-14 affinity column chromatography and mass spectrometry. Both LGALS-11 and LGALS-14 bound more putative protein targets in the adult stage of H. contortus (43 proteins) when compared to the larval stage (two proteins). Of the 43 proteins identified in the adult stage, 34 and 35 proteins were bound by LGALS-11 and LGALS-14, respectively, with 26 proteins binding to both galectins. Interestingly, hematophagous stage-specific sperm-coating protein and zinc metalloprotease (M13), which are known vaccine candidates, were identified as putative ligands of both LGALS-11 and LGALS-14. The identification of glycoproteins of H. contortus by LGALS-11 and LGALS-14 provide new insights into host-parasite interactions and the potential for developing new interventions.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: PeerJ Año: 2018 Tipo del documento: Article País de afiliación: Australia

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: PeerJ Año: 2018 Tipo del documento: Article País de afiliación: Australia