Immobilization of D-Amino Acid Oxidase (DAAO) Enzyme on Hybrid Mesoporous MCF, SBA-15 and MCM-41 Nanomaterial.
J Nanosci Nanotechnol
; 17(2): 947-53, 2017 Feb.
Article
en En
| MEDLINE
| ID: mdl-29671483
ABSTRACT
Hybrid mesoporous materials as carriers for immobilization of D-amino acid oxidase (DAAO) were prepared via three steps:
(i) hydrothermal synthesis of nanoporous MCF, SBA-15 and MCM-41 powders, (ii) functionalization with 3-aminopropyltriethoxysilane (APTES) by post-synthesis grafting; and (iii) activation with glutardialdehyde. The resulting mesostructured solids were characterized by various techniques XRD, IR, TGA-DTA and N2 adsorptiondesorption (BET). The characterization results indicated that these materials still maintained their structure after functionalization. IR data and TGA-DTA analysis demonstrated the existence of amine functional groups on the surface of APTES-functionalized samples. The DAAO immobilized on these materials exhibited higher catalytic activity and stability of enzyme for conversion of cephalosporin C (CPC) as compared to those of the non-functionalized ones. The catalytic activity and stability of enzyme decreased in the order MCF > SBA-15 > MCM-41.Palabras clave
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Compuestos de Silicona
/
D-Aminoácido Oxidasa
/
Nanoestructuras
/
Enzimas Inmovilizadas
Idioma:
En
Revista:
J Nanosci Nanotechnol
Año:
2017
Tipo del documento:
Article