Reconstruction From Multiple Particles for 3D Isotropic Resolution in Fluorescence Microscopy.
IEEE Trans Med Imaging
; 37(5): 1235-1246, 2018 05.
Article
en En
| MEDLINE
| ID: mdl-29727286
ABSTRACT
The imaging of proteins within macromolecular complexes has been limited by the low axial resolution of optical microscopes. To overcome this problem, we propose a novel computational reconstruction method that yields isotropic resolution in fluorescence imaging. The guiding principle is to reconstruct a single volume from the observations of multiple rotated particles. Our new operational framework detects particles, estimates their orientation, and reconstructs the final volume. The main challenge comes from the absence of initial template and a priori knowledge about the orientations. We formulate the estimation as a blind inverse problem, and propose a block-coordinate stochastic approach to solve the associated non-convex optimization problem. The reconstruction is performed jointly in multiple channels. We demonstrate that our method is able to reconstruct volumes with 3D isotropic resolution on simulated data. We also perform isotropic reconstructions from real experimental data of doubly labeled purified human centrioles. Our approach revealed the precise localization of the centriolar protein Cep63 around the centriole microtubule barrel. Overall, our method offers new perspectives for applications in biology that require the isotropic mapping of proteins within macromolecular assemblies.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas
/
Imagenología Tridimensional
/
Microscopía Fluorescente
Tipo de estudio:
Clinical_trials
Límite:
Humans
Idioma:
En
Revista:
IEEE Trans Med Imaging
Año:
2018
Tipo del documento:
Article