X-ray spectroscopy and imaging of selenium in living systems.
Biochim Biophys Acta Gen Subj
; 1862(11): 2383-2392, 2018 Nov.
Article
en En
| MEDLINE
| ID: mdl-29729308
ABSTRACT
BACKGROUND:
Selenium is an essential element with a rich and varied chemistry in living organisms. It plays a variety of important roles ranging from being essential in enzymes that are critical for redox homeostasis to acting as a deterrent for herbivory in hyperaccumulating plants. Despite its importance there are many open questions, especially related to its chemistry in situ within living organisms. SCOPE OF REVIEW This review discusses X-ray spectroscopy and imaging of selenium in biological samples, with an emphasis on the methods, and in particular the techniques of X-ray absorption spectroscopy (XAS) and X-ray fluorescence imaging (XFI). We discuss the experimental methods and capabilities of XAS and XFI, and review their advantages and their limitations. A perspective on future possibilities and next-generation of experiments is also provided. MAJORCONCLUSIONS:
XAS and XFI provide powerful probes of selenium chemistry, together with unique in situ capabilities. The opportunities and capabilities of the next generation of advanced X-ray spectroscopy experiments are particularly exciting. GENERALSIGNIFICANCE:
XAS and XFI provide versatile tools that are generally applicable to any element with a convenient X-ray absorption edge, suitable for investigating complex systems essentially without pre-treatment.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Idioma:
En
Revista:
Biochim Biophys Acta Gen Subj
Año:
2018
Tipo del documento:
Article
País de afiliación:
Canadá