Hypoxia-Induced Reporter Genes with Different Half-Lives.
Methods Mol Biol
; 1790: 113-125, 2018.
Article
en En
| MEDLINE
| ID: mdl-29858787
ABSTRACT
The utility of reporter genes has gained significant momentum over the last three decades. Reporter genes are used to understand the transcriptional activity of a gene both in vitro and in vivo, and in pathway analysis and drug screening for diseases involving protozoan parasites, and in anti-cancer drug developments. Here, using a human prostate cancer xenograft model (PC3), we describe a method to construct and validate hypoxia reporter genes with different half-lives. Using molecular biology and optical imaging techniques, we have validated the expression of long half-life enhanced green fluorescence protein (EGFP) expression and short half-life luciferase gene expression to report on the spatial and temporal evolution of hypoxia in vivo.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Neoplasias de la Próstata
/
Regulación Neoplásica de la Expresión Génica
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Genes Reporteros
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Proteínas Fluorescentes Verdes
/
Imagen Molecular
/
Luciferasas
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Hipoxia
Límite:
Animals
/
Humans
/
Male
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2018
Tipo del documento:
Article
País de afiliación:
Estados Unidos