Experimental and bioinformatic approach to identifying antigenic epitopes in human &#945;- and ß-enolases.

Pietkiewicz, Jadwiga; Danielewicz, Regina; Bednarz-Misa, Iwona S; Ceremuga, Ireneusz; Wisniewski, Jerzy; Mierzchala-Pasierb, Magdalena; Bronowicka-Szydelko, Agnieszka; Ziomek, Edmund; Gamian, Andrzej

Experimental and bioinformatic approach to identifying antigenic epitopes in human α- and ß-enolases.

Pietkiewicz, Jadwiga; Danielewicz, Regina; Bednarz-Misa, Iwona S; Ceremuga, Ireneusz; Wisniewski, Jerzy; Mierzchala-Pasierb, Magdalena; Bronowicka-Szydelko, Agnieszka; Ziomek, Edmund; Gamian, Andrzej.

Afiliación

Pietkiewicz J; Department of Medical Biochemistry, Wroclaw Medical University,Chalubinskiego 10, 50-368 Wroclaw, Poland.
Danielewicz R; Department of Medical Biochemistry, Wroclaw Medical University,Chalubinskiego 10, 50-368 Wroclaw, Poland.
Bednarz-Misa IS; Department of Medical Biochemistry, Wroclaw Medical University,Chalubinskiego 10, 50-368 Wroclaw, Poland.
Ceremuga I; Department of Medical Biochemistry, Wroclaw Medical University,Chalubinskiego 10, 50-368 Wroclaw, Poland.
Wisniewski J; Department of Medical Biochemistry, Wroclaw Medical University,Chalubinskiego 10, 50-368 Wroclaw, Poland.
Mierzchala-Pasierb M; Department of Medical Biochemistry, Wroclaw Medical University,Chalubinskiego 10, 50-368 Wroclaw, Poland.
Bronowicka-Szydelko A; Department of Medical Biochemistry, Wroclaw Medical University,Chalubinskiego 10, 50-368 Wroclaw, Poland.
Ziomek E; Wroclaw Research Center, Stablowicka 147, 50-066 Wroclaw, Poland.
Gamian A; Department of Medical Biochemistry, Wroclaw Medical University,Chalubinskiego 10, 50-368 Wroclaw, Poland.

Biochem Biophys Rep ; 15: 25-32, 2018 Sep.

Article en En | MEDLINE | ID: mdl-29922723

Palabras clave

AP, alkaline phosphatase; BSA, bovine serum albumin; Cross-reactivity; ELISA, enzyme-linked immunosorbent assay; ESI, electrospray injection; Enolase purification; Epitope prediction; HRP, horse radish peroxidase; IgG, immunoglobulin G; LC, liquid chromatography; MS, mass spectrometry; Mass spectrometry; MeOH, methanol; OPD, ortho-phenylenediamine; PAGE, polyacrylamide gel electrophoresis; PBS, phosphate buffered saline; PMSF, phenylmethylsulfonyl fluoride; SDS, sodium dodecylsulfate; Specific antibodies; TBST, 20â¯mM Tris, pH 7.4, 150â¯mM NaCl, 0.05% Tween-20; UPLC-Q-TOF-MS, ultrapressure liquid chromatography, quadrupole-time-of-flight mass spectrometer; WB, western blotting; pNPP, para-nitrophenyl phosphate

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Idioma: En Revista: Biochem Biophys Rep Año: 2018 Tipo del documento: Article País de afiliación: Polonia

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