Experimental and bioinformatic approach to identifying antigenic epitopes in human α- and ß-enolases.
Biochem Biophys Rep
; 15: 25-32, 2018 Sep.
Article
en En
| MEDLINE
| ID: mdl-29922723
AP, alkaline phosphatase; BSA, bovine serum albumin; Cross-reactivity; ELISA, enzyme-linked immunosorbent assay; ESI, electrospray injection; Enolase purification; Epitope prediction; HRP, horse radish peroxidase; IgG, immunoglobulin G; LC, liquid chromatography; MS, mass spectrometry; Mass spectrometry; MeOH, methanol; OPD, ortho-phenylenediamine; PAGE, polyacrylamide gel electrophoresis; PBS, phosphate buffered saline; PMSF, phenylmethylsulfonyl fluoride; SDS, sodium dodecylsulfate; Specific antibodies; TBST, 20â¯mM Tris, pH 7.4, 150â¯mM NaCl, 0.05% Tween-20; UPLC-Q-TOF-MS, ultrapressure liquid chromatography, quadrupole-time-of-flight mass spectrometer; WB, western blotting; pNPP, para-nitrophenyl phosphate
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Idioma:
En
Revista:
Biochem Biophys Rep
Año:
2018
Tipo del documento:
Article
País de afiliación:
Polonia