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Human mesenchymal-stem-cells-derived exosomes are important in enhancing porcine islet resistance to hypoxia.
Nie, Wei; Ma, Xiaoqian; Yang, Cejun; Chen, Zeyi; Rong, Pengfei; Wu, Minghua; Jiang, Jianhui; Tan, Mengqun; Yi, Shounan; Wang, Wei.
Afiliación
  • Nie W; Cell Transplantation and Gene Therapy Institute, The Third Xiang Ya Hospital, Central South University, Changsha, Hunan, China.
  • Ma X; Engineering and Technology Research Center for Xenotransplantation of Hunan Province, Changsha, China.
  • Yang C; Cell Transplantation and Gene Therapy Institute, The Third Xiang Ya Hospital, Central South University, Changsha, Hunan, China.
  • Chen Z; Engineering and Technology Research Center for Xenotransplantation of Hunan Province, Changsha, China.
  • Rong P; Cell Transplantation and Gene Therapy Institute, The Third Xiang Ya Hospital, Central South University, Changsha, Hunan, China.
  • Wu M; Engineering and Technology Research Center for Xenotransplantation of Hunan Province, Changsha, China.
  • Jiang J; Engineering and Technology Research Center for Xenotransplantation of Hunan Province, Changsha, China.
  • Tan M; Cell Transplantation and Gene Therapy Institute, The Third Xiang Ya Hospital, Central South University, Changsha, Hunan, China.
  • Yi S; Cell Transplantation and Gene Therapy Institute, The Third Xiang Ya Hospital, Central South University, Changsha, Hunan, China.
  • Wang W; State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, China.
Xenotransplantation ; 25(5): e12405, 2018 09.
Article en En | MEDLINE | ID: mdl-29932262
ABSTRACT

BACKGROUND:

Hypoxia-induced damage is one of the key factors associated with islet graft dysfunction. Mesenchymal stem cells (MSCs) could be used to enhance the therapeutic effect of islet transplantation due to their paracrine potential such as exosomes. In this study, we investigated whether exosomes from human umbilical cord-derived MSC-conditioned medium (hu-MSC-CM) could increase the survival and function of neonatal porcine islet cell clusters (NICCs) exposed to hypoxia.

METHODS:

Neonatal porcine islet cell clusters were cultured with hu-MSC-CM, with or without exosomes, and native medium RPMI-1640 (Control) under hypoxic conditions (1% O2 ). The effects of exosomes on NICCs viability and function in vitro were examined by FACS, the Loops system, and the Extracellular Flux assay, respectively.

RESULTS:

Compared with NICCs cultured in RPMI-1640 medium and hu-MSC-CM without exosomes, the survival ratio, viability, and function increased in NICCs cultured in hu-MSC-CM with exosomes.

CONCLUSIONS:

This study found that hu-MSC-CM could protect NICCs from hypoxia-induced dysfunction, and exosomes played an important role in hypoxic resistance, suggesting a potential strategy to improve islet transplantation outcomes.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Islotes Pancreáticos / Exosomas / Células Madre Mesenquimatosas / Hipoxia Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Xenotransplantation Asunto de la revista: TRANSPLANTE Año: 2018 Tipo del documento: Article País de afiliación: China
Texto completo
Imprimir
XML
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Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Islotes Pancreáticos / Exosomas / Células Madre Mesenquimatosas / Hipoxia Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Xenotransplantation Asunto de la revista: TRANSPLANTE Año: 2018 Tipo del documento: Article País de afiliación: China