Antimicrobial efficiency and stability of two decontamination solutions.
Cell Tissue Bank
; 19(4): 581-589, 2018 Dec.
Article
en En
| MEDLINE
| ID: mdl-30062597
ABSTRACT
Two decontamination solutions, commercially produced BASEâ¢128 and laboratory decontamination solution (LDS), with analogous content of antibiotic and antimycotic agents, were compared in their antimicrobial efficiency and stability (pH and osmolarity). Both solutions were compared immediately after thawing aliquots frozen for 1, 3 or 6 months. Agar well diffusion method was used to test their antimicrobial efficiency against five human pathogens Staphylococcus aureus, Pseudomonas aeruginosa, Proteus mirabilis, Escherichia coli and Enterococcus faecalis. The difference in the inhibition of growth between the two decontamination solutions was mostly not statistically significant, with few exceptions. The most pronounced difference between the LDS and BASEâ¢128 was observed in their decontamination efficacy against E. coli and E. faecalis, where the LDS showed to be more efficient than BASEâ¢128. The osmolarity value of LDS decreased with cold-storage, the osmolarity values of the BASEâ¢128 could not be measured as they were below the range of the osmometer. Slight changes were found in pH of the less stable LDS solution, whose pH increased from initial value 7.36 ± 0.07 to 7.72 ± 0.19 after 6 m-storage. We verified that BASEâ¢128 and LDS are similarly efficient in elimination of possible placental bacterial contaminants and may be used for decontamination of various tissues.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Descontaminación
/
Antiinfecciosos
Idioma:
En
Revista:
Cell Tissue Bank
Asunto de la revista:
HISTOLOGIA
/
TRANSPLANTE
Año:
2018
Tipo del documento:
Article
País de afiliación:
República Checa