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Single-DNA electron spin resonance spectroscopy in aqueous solutions.
Shi, Fazhan; Kong, Fei; Zhao, Pengju; Zhang, Xiaojun; Chen, Ming; Chen, Sanyou; Zhang, Qi; Wang, Mengqi; Ye, Xiangyu; Wang, Zhecheng; Qin, Zhuoyang; Rong, Xing; Su, Jihu; Wang, Pengfei; Qin, Peter Z; Du, Jiangfeng.
Afiliación
  • Shi F; CAS Key Laboratory of Microscale Magnetic Resonance and Department of Modern Physics, University of Science and Technology of China (USTC), Hefei, China.
  • Kong F; Hefei National Laboratory for Physical Sciences at the Microscale, USTC, Hefei, China.
  • Zhao P; Synergetic Innovation Center of Quantum Information and Quantum Physics, USTC, Hefei, China.
  • Zhang X; CAS Key Laboratory of Microscale Magnetic Resonance and Department of Modern Physics, University of Science and Technology of China (USTC), Hefei, China.
  • Chen M; Hefei National Laboratory for Physical Sciences at the Microscale, USTC, Hefei, China.
  • Chen S; Synergetic Innovation Center of Quantum Information and Quantum Physics, USTC, Hefei, China.
  • Zhang Q; CAS Key Laboratory of Microscale Magnetic Resonance and Department of Modern Physics, University of Science and Technology of China (USTC), Hefei, China.
  • Wang M; Synergetic Innovation Center of Quantum Information and Quantum Physics, USTC, Hefei, China.
  • Ye X; Department of Chemistry, University of Southern California, Los Angeles, CA, USA.
  • Wang Z; CAS Key Laboratory of Microscale Magnetic Resonance and Department of Modern Physics, University of Science and Technology of China (USTC), Hefei, China.
  • Qin Z; Synergetic Innovation Center of Quantum Information and Quantum Physics, USTC, Hefei, China.
  • Rong X; CAS Key Laboratory of Microscale Magnetic Resonance and Department of Modern Physics, University of Science and Technology of China (USTC), Hefei, China.
  • Su J; Synergetic Innovation Center of Quantum Information and Quantum Physics, USTC, Hefei, China.
  • Wang P; CAS Key Laboratory of Microscale Magnetic Resonance and Department of Modern Physics, University of Science and Technology of China (USTC), Hefei, China.
  • Qin PZ; Synergetic Innovation Center of Quantum Information and Quantum Physics, USTC, Hefei, China.
  • Du J; CAS Key Laboratory of Microscale Magnetic Resonance and Department of Modern Physics, University of Science and Technology of China (USTC), Hefei, China.
Nat Methods ; 15(9): 697-699, 2018 09.
Article en En | MEDLINE | ID: mdl-30082898
ABSTRACT
Magnetic resonance spectroscopy of single biomolecules under near-physiological conditions could substantially advance understanding of their biological function, but this approach remains very challenging. Here we used nitrogen-vacancy centers in diamonds to detect electron spin resonance spectra of individual, tethered DNA duplexes labeled with a nitroxide spin label in aqueous buffer solutions at ambient temperatures. This work paves the way for magnetic resonance studies on single biomolecules and their intermolecular interactions in native-like environments.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: ADN / Espectroscopía de Resonancia por Spin del Electrón / Imagen Individual de Molécula Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2018 Tipo del documento: Article País de afiliación: China
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: ADN / Espectroscopía de Resonancia por Spin del Electrón / Imagen Individual de Molécula Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2018 Tipo del documento: Article País de afiliación: China