Green synthesis of cellulose nanofibers using immobilized cellulase.
Carbohydr Polym
; 205: 255-260, 2019 Feb 01.
Article
en En
| MEDLINE
| ID: mdl-30446102
ABSTRACT
Isolation of cellulose nanofibers (CNF) using cellulase immobilized on cheap and easily formed polymeric gel disks is discussed. Such gel disks based on carrageenan gel coated with hyperbranched polyamidoamine that can covalently bind to cellulase through glutaraldehyde spacer. Thermal and mechanical stability of the coated gel disks were significantly improved. Free and immobilized cellulase exhibited maximum activities at 50 °C and pH 5. However, immobilized cellulase exhibited broader temperature stability than in the free form. Additionally, immobilized cellulase gel disks can be easily separated and reused with great reusability capacity of about 85% of the initial activity after six cycles. Immobilized cellulase was capable to disintegrate cellulose fibers providing nanofibers with diameter of 15-35 nm and several micrometers long. Overall, enhanced thermal stability and reusability of immobilized cellulase pave the way for its use in industrial production of CNF that can be applicable for biomedical and food packaging applications.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Celulasa
/
Celulosa
/
Enzimas Inmovilizadas
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Tecnología Química Verde
/
Nanofibras
Idioma:
En
Revista:
Carbohydr Polym
Año:
2019
Tipo del documento:
Article