Visualization and Quantitation of Phase-Separated Droplet Formation by Human HP1α.
Methods Enzymol
; 611: 51-66, 2018.
Article
en En
| MEDLINE
| ID: mdl-30471698
ABSTRACT
The ability of the heterochromatin protein-1 (HP1) to phase separate into droplets suggests new mechanisms of gene organization in the cell nucleus. An accumulating body of work suggests that other nuclear proteins also display phase separation behaviors in vitro. To understand the mechanistic and biological significance of such droplet formation a rigorous biophysical characterization of this behavior is necessary. Herein we describe procedures for imaging HP1 droplets by brightfield microscopy, and two methods to quantify phase separation.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Espectrofotometría
/
Proteínas Cromosómicas no Histona
/
Transición de Fase
/
Microscopía
Límite:
Humans
Idioma:
En
Revista:
Methods Enzymol
Año:
2018
Tipo del documento:
Article
País de afiliación:
Estados Unidos