Faster, sharper, and deeper: structured illumination microscopy for biological imaging.

Wu, Yicong; Shroff, Hari

Wu, Yicong; Shroff, Hari.

Afiliación

Wu Y; Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA. yicong.wu@nih.gov.
Shroff H; Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA.

Nat Methods ; 15(12): 1011-1019, 2018 12.

Article en En | MEDLINE | ID: mdl-30478322

ABSTRACT

ABSTRACT

Structured illumination microscopy (SIM) allows rapid, super-resolution (SR) imaging in live specimens. We review recent technical advances in SR-SIM, with emphasis on imaging speed, resolution, and depth. Since its introduction decades ago, the technique has grown to offer myriad implementations, each with its own strengths and weaknesses. We discuss these, aiming to provide a practical guide for biologists and to highlight which approach is best suited to a given application.

Asunto(s)

Procesamiento de Imagen Asistido por Computador/métodos; Iluminación/instrumentación; Microscopía Fluorescente/métodos; Modelos Biológicos; Humanos

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Procesamiento de Imagen Asistido por Computador / Iluminación / Microscopía Fluorescente / Modelos Biológicos Límite: Humans Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos

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