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A multiplexable assay for screening antibiotic lethality against drug-tolerant bacteria.
Stokes, Jonathan M; Gutierrez, Arnaud; Lopatkin, Allison J; Andrews, Ian W; French, Shawn; Matic, Ivan; Brown, Eric D; Collins, James J.
Afiliación
  • Stokes JM; Institute for Medical Engineering & Science, Department of Biological Engineering, and Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Gutierrez A; Infectious Disease and Microbiome Program, Broad Institute, Cambridge, MA, USA.
  • Lopatkin AJ; Institute for Medical Engineering & Science, Department of Biological Engineering, and Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Andrews IW; Infectious Disease and Microbiome Program, Broad Institute, Cambridge, MA, USA.
  • French S; Inserm U1001, Faculté de Médecine Paris Descartes, Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
  • Matic I; Institute for Medical Engineering & Science, Department of Biological Engineering, and Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Brown ED; Infectious Disease and Microbiome Program, Broad Institute, Cambridge, MA, USA.
  • Collins JJ; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA.
Nat Methods ; 16(4): 303-306, 2019 04.
Article en En | MEDLINE | ID: mdl-30858599
ABSTRACT
Antibiotic screens typically rely on growth inhibition to characterize compound bioactivity-an approach that cannot be used to assess the bactericidal activity of antibiotics against bacteria in drug-tolerant states. To address this limitation, we developed a multiplexed assay that uses metabolism-sensitive staining to report on the killing of antibiotic-tolerant bacteria. This method can be used with diverse bacterial species and applied to genome-scale investigations to identify therapeutic targets against tolerant pathogens.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Bacterias / Pruebas de Sensibilidad Microbiana / Farmacorresistencia Bacteriana / Escherichia coli / Antibacterianos Tipo de estudio: Diagnostic_studies / Screening_studies Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Bacterias / Pruebas de Sensibilidad Microbiana / Farmacorresistencia Bacteriana / Escherichia coli / Antibacterianos Tipo de estudio: Diagnostic_studies / Screening_studies Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos