Protein fibrillogenesis model tracked by its intrinsic time-resolved emission spectra.
Methods Appl Fluoresc
; 7(3): 035003, 2019 May 16.
Article
en En
| MEDLINE
| ID: mdl-30986770
ABSTRACT
The excited-state kinetics of the fluorescence of tyrosine in a de novo protein fibrillogenesis model was investigated as a potential tool for monitoring protein fibre formation and complexation with glucose (glycation). In stark contrast to insulin the time-resolved emission spectra (TRES) recorded over the period of 700 hours in buffered solutions of the model with and without glucose revealed no apparent changes in Tyr fluorescence responses. This indicates the stability of the model and provides a measurement-supported basis for its use as a reference material in fluorescence studies of protein aggregation.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Péptidos
/
Proteínas Amiloidogénicas
Idioma:
En
Revista:
Methods Appl Fluoresc
Año:
2019
Tipo del documento:
Article
País de afiliación:
Reino Unido