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Nanoscale magnetic imaging of ferritins in a single cell.
Wang, Pengfei; Chen, Sanyou; Guo, Maosen; Peng, Shijie; Wang, Mengqi; Chen, Ming; Ma, Wenchao; Zhang, Rui; Su, Jihu; Rong, Xing; Shi, Fazhan; Xu, Tao; Du, Jiangfeng.
Afiliación
  • Wang P; CAS Key Laboratory of Microscale Magnetic Resonance and Department of Modern Physics, University of Science and Technology of China, Hefei 230026, China.
  • Chen S; Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230026, China.
  • Guo M; Synergetic Innovation Center of Quantum Information and Quantum Physics, University of Science and Technology of China, Hefei 230026, China.
  • Peng S; CAS Key Laboratory of Microscale Magnetic Resonance and Department of Modern Physics, University of Science and Technology of China, Hefei 230026, China.
  • Wang M; Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230026, China.
  • Chen M; Synergetic Innovation Center of Quantum Information and Quantum Physics, University of Science and Technology of China, Hefei 230026, China.
  • Ma W; CAS Key Laboratory of Microscale Magnetic Resonance and Department of Modern Physics, University of Science and Technology of China, Hefei 230026, China.
  • Zhang R; Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230026, China.
  • Su J; Synergetic Innovation Center of Quantum Information and Quantum Physics, University of Science and Technology of China, Hefei 230026, China.
  • Rong X; CAS Key Laboratory of Microscale Magnetic Resonance and Department of Modern Physics, University of Science and Technology of China, Hefei 230026, China.
  • Shi F; Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230026, China.
  • Xu T; Synergetic Innovation Center of Quantum Information and Quantum Physics, University of Science and Technology of China, Hefei 230026, China.
  • Du J; CAS Key Laboratory of Microscale Magnetic Resonance and Department of Modern Physics, University of Science and Technology of China, Hefei 230026, China.
Sci Adv ; 5(4): eaau8038, 2019 04.
Article en En | MEDLINE | ID: mdl-30989109
ABSTRACT
The in situ measurement of the distribution of biomolecules inside a cell is one of the important goals in life science. Among various imaging techniques, magnetic imaging (MI) based on the nitrogen-vacancy (NV) center in diamond provides a powerful tool for the biomolecular research, while the nanometer-scale MI of intracellular proteins remains a challenge. Here, we use ferritin as a demonstration to realize the MI of endogenous proteins in a single cell using the NV center as the sensor. With the scanning, intracellular ferritins are imaged with a spatial resolution of ca. 10 nm, and ferritin-containing organelles are colocalized by correlative MI and electron microscopy. The approach paves the way for nanoscale MI of intracellular proteins.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Imagen Molecular / Ferritinas / Análisis de la Célula Individual / Fenómenos Magnéticos Límite: Humans Idioma: En Revista: Sci Adv Año: 2019 Tipo del documento: Article País de afiliación: China
Texto completo
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Imagen Molecular / Ferritinas / Análisis de la Célula Individual / Fenómenos Magnéticos Límite: Humans Idioma: En Revista: Sci Adv Año: 2019 Tipo del documento: Article País de afiliación: China