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An approach for visualizing the spatial metabolome of an entire plant root system inspired by the Swiss-rolling technique.
Velickovic, Dusan; Chu, Rosalie K; Myers, Gabriel L; Ahkami, Amir H; Anderton, Christopher R.
Afiliación
  • Velickovic D; Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington.
  • Chu RK; Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington.
  • Myers GL; Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington.
  • Ahkami AH; Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington.
  • Anderton CR; Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington.
J Mass Spectrom ; 55(4): e4363, 2020 Apr.
Article en En | MEDLINE | ID: mdl-31018010
The spatial configuration and morphology of roots are commonly monitored for a better understanding of plant health and development. However, this approach provides minimal details about the biochemistry regulating the observable traits. Therefore, the ability to metabolically map the entire root structure would be of major value. Here, we developed a sample preparation approach that enables imaging of the entire root within a restricted space (width of microscope slide), which was influenced by the Swiss-rolling technique. We were able to image and confidently identify molecules along the entire root structure from rolled-root tissue sections using multiple spatially resolved mass spectrometry approaches.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Raíces de Plantas / Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción / Metaboloma / Metabolómica Idioma: En Revista: J Mass Spectrom Asunto de la revista: BIOQUIMICA Año: 2020 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Raíces de Plantas / Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción / Metaboloma / Metabolómica Idioma: En Revista: J Mass Spectrom Asunto de la revista: BIOQUIMICA Año: 2020 Tipo del documento: Article