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Evaluation of an Engineered Hybrid Matrix for Bone Regeneration via Endochondral Ossification.
Mikael, Paiyz E; Golebiowska, Aleksandra A; Xin, Xiaonan; Rowe, David W; Nukavarapu, Syam P.
Afiliación
  • Mikael PE; Department of Materials Science, & Engineering, University of Connecticut, Storrs, CT, 06269, USA.
  • Golebiowska AA; Department of Biomedical Engineering, University of Connecticut, 260 Glenbrook Road, Unit 3247, Storrs, CT, 06269, USA.
  • Xin X; Center for Regenerative Medicine and Skeletal Development, University of Connecticut Health, Farmington, CT, 06032, USA.
  • Rowe DW; Center for Regenerative Medicine and Skeletal Development, University of Connecticut Health, Farmington, CT, 06032, USA.
  • Nukavarapu SP; Department of Materials Science, & Engineering, University of Connecticut, Storrs, CT, 06269, USA. syam.nukavarapu@uconn.edu.
Ann Biomed Eng ; 48(3): 992-1005, 2020 Mar.
Article en En | MEDLINE | ID: mdl-31037444
ABSTRACT
Despite its regenerative ability, long and segmental bone defect repair remains a significant orthopedic challenge. Conventional tissue engineering efforts induce bone formation through intramembranous ossification (IO) which limits vascular formation and leads to poor bone regeneration. To overcome this challenge, a novel hybrid matrix comprised of a load-bearing polymer template and a gel phase is designed and assessed for bone regeneration. Our previous studies developed a synthetic ECM, hyaluronan (HA)-fibrin (FB), that is able to mimic cartilage-mediated bone formation in vitro. In this study, the well-characterized HA-FB hydrogel is combined with a biodegradable polymer template to form a hybrid matrix. In vitro evaluation of the matrix showed cartilage template formation, cell recruitment and recruited cell osteogenesis, essential stages in endochondral ossification. A transgenic reporter-mouse critical-defect model was used to evaluate the bone healing potential of the hybrid matrix in vivo. The results demonstrated host cell recruitment into the hybrid matrix that led to new bone formation and subsequent remodeling of the mineralization. Overall, the study developed and evaluated a novel load-bearing graft system for bone regeneration via endochondral ossification.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Osteogénesis / Cráneo / Regeneración Ósea / Ingeniería de Tejidos / Células Madre Mesenquimatosas Límite: Animals / Humans Idioma: En Revista: Ann Biomed Eng Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Osteogénesis / Cráneo / Regeneración Ósea / Ingeniería de Tejidos / Células Madre Mesenquimatosas Límite: Animals / Humans Idioma: En Revista: Ann Biomed Eng Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos