Immunofluorescence Labeling of Nuclear Receptor Expression in Formalin-Fixed, Paraffin-Embedded Tissue.

Lightbody, Elizabeth D; Nicol, Christopher J B

Lightbody, Elizabeth D; Nicol, Christopher J B.

Afiliación

Lightbody ED; Department of Pathology and Molecular Medicine, Queen's University, Kingston, ON, Canada.
Nicol CJB; Division of Cancer Biology and Genetics, Cancer Research Institute, Queen's University, Kingston, ON, Canada.

Methods Mol Biol ; 1966: 101-105, 2019.

Article en En | MEDLINE | ID: mdl-31041741

ABSTRACT

ABSTRACT

Immunofluorescent staining (IF) uses antigen-antibody complexes tagged with fluorochromes to observe the expression of proteins within a tissue sample. Multiple groups have described optimized methods to visualize several proteins simultaneously within the same tissue section using immunofluorescence in both mouse and human FFPE tissues. Our group routinely uses an optimized protocol described here to examine nuclear receptor expression in experimental samples from conditional knockout in vivo studies.

Asunto(s)

Técnica del Anticuerpo Fluorescente/métodos; Expresión Génica; Adhesión en Parafina; Receptores Citoplasmáticos y Nucleares/análisis; Animales; Formaldehído; Humanos; Ratones; Receptores Citoplasmáticos y Nucleares/genética

Palabras clave

FFPE; Immunofluorescent labeling; Nuclear receptor

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Expresión Génica / Técnica del Anticuerpo Fluorescente / Adhesión en Parafina / Receptores Citoplasmáticos y Nucleares Límite: Animals / Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2019 Tipo del documento: Article País de afiliación: Canadá

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