Past, Present, and Future of Neuronal Models In Vitro.
Adv Neurobiol
; 22: 3-17, 2019.
Article
en En
| MEDLINE
| ID: mdl-31073930
ABSTRACT
Over the past century, robust methods were developed that enable the isolation, culture, and dynamic observation of mammalian neuronal networks in vitro. But even if neuronal culture cannot yet fully recapitulate the normal brain, the knowledge that has been acquired from these surrogate in vitro models is invaluable. Indeed, neuronal culture has continued to propel basic neuroscience research, proving that in vitro systems have legitimacy when it comes to studying either the healthy or diseased human brain. Furthermore, scientific advancement typically parallels technical refinements in the field. A pertinent example is that a collective drive in the field of neuroscience to better understand the development, organization, and emergent properties of neuronal networks is being facilitated by progressive advances in micro-electrode array (MEA) technology. In this chapter, we briefly review the emergence of neuronal cell culture as a technique, the current trends in human stem cell-based modeling, and the technologies used to monitor neuronal communication. We conclude by highlighting future prospects that are evolving specifically out of the combination of human neuronal models and MEA technology.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Técnicas In Vitro
/
Técnicas de Cultivo de Célula
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Modelos Neurológicos
/
Neuronas
Límite:
Animals
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Humans
Idioma:
En
Revista:
Adv Neurobiol
Año:
2019
Tipo del documento:
Article
País de afiliación:
Países Bajos