Long-term outcomes of patch tracheoplasty using collagenous tissue membranes (biosheets) produced by in-body tissue architecture in a beagle model.
Surg Today
; 49(11): 958-964, 2019 Nov.
Article
en En
| MEDLINE
| ID: mdl-31098758
ABSTRACT
PURPOSE:
Although various artificial tracheas have been developed, none have proven satisfactory for clinical use. In-body tissue architecture (IBTA) has enabled us to produce collagenous tissues with a wide range of shapes and sizes to meet the needs of individual recipients. In the present study, we investigated the long-term outcomes of patch tracheoplasty using an IBTA-induced collagenous tissue membrane ("biosheet") in a beagle model.METHODS:
Nine adult female beagles were used. Biosheets were prepared by embedding cylindrical molds assembled with a silicone rod and a slitting pipe into dorsal subcutaneous pouches for 2 months. The sheets were then implanted by patch tracheoplasty. An endoscopic evaluation was performed after 1, 3, or 12 months. The implanted biosheets were harvested for a histological evaluation at the same time points.RESULTS:
All animals survived the study. At 1 month after tracheoplasty, the anastomotic parts and internal surface of the biosheets were smooth with ciliated columnar epithelium, which regenerated into the internal surface of the biosheet. The chronological spread of chondrocytes into the biosheet was observed at 3 and 12 months.CONCLUSIONS:
Biosheets showed excellent performance as a scaffold for trachea regeneration with complete luminal epithelium and partial chondrocytes in a 1-year beagle implantation model of patch tracheoplasty.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Tráquea
/
Estenosis Traqueal
/
Materiales Biocompatibles
/
Procedimientos de Cirugía Plástica
/
Ingeniería de Tejidos
/
Membranas Artificiales
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Surg Today
Año:
2019
Tipo del documento:
Article
País de afiliación:
Japón