Biochemical Characterization of Full-Length Oncogenic BRAF<sup>V600E</sup> together with Molecular Dynamics Simulations Provide Insight into the Activation and Inhibition Mechanisms of RAF Kinases.

Cope, Nicholas; Novak, Borna; Candelora, Christine; Wong, Kenneth; Cavallo, Maria; Gunderwala, Amber; Liu, Zhiwei; Li, Yana; Wang, Zhihong

Biochemical Characterization of Full-Length Oncogenic BRAF^V600E together with Molecular Dynamics Simulations Provide Insight into the Activation and Inhibition Mechanisms of RAF Kinases.

Cope, Nicholas; Novak, Borna; Candelora, Christine; Wong, Kenneth; Cavallo, Maria; Gunderwala, Amber; Liu, Zhiwei; Li, Yana; Wang, Zhihong.

Afiliación

Cope N; Department of Chemistry and Biochemistry, University of the Sciences, Philadelphia, PA, 19104, USA.
Novak B; Department of Chemistry and Biochemistry, University of the Sciences, Philadelphia, PA, 19104, USA.
Candelora C; Department of Chemistry and Biochemistry, University of the Sciences, Philadelphia, PA, 19104, USA.
Wong K; Department of Chemistry and Biochemistry, University of the Sciences, Philadelphia, PA, 19104, USA.
Cavallo M; Department of Chemistry and Biochemistry, University of the Sciences, Philadelphia, PA, 19104, USA.
Gunderwala A; Department of Chemistry and Biochemistry, University of the Sciences, Philadelphia, PA, 19104, USA.
Liu Z; Department of Chemistry and Biochemistry, University of the Sciences, Philadelphia, PA, 19104, USA.
Li Y; Eukaryotic Tissue Culture Facility, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA.
Wang Z; Department of Chemistry and Biochemistry, University of the Sciences, Philadelphia, PA, 19104, USA.

Chembiochem ; 20(22): 2850-2861, 2019 11 18.

Article en En | MEDLINE | ID: mdl-31152574

Asunto(s)

Proteínas Proto-Oncogénicas B-raf/química; Activadores de Enzimas/metabolismo; Células HEK293; Humanos; Imidazoles/metabolismo; Cinética; Simulación de Dinámica Molecular; Mutación; Oximas/metabolismo; Fosforilación/genética; Unión Proteica; Inhibidores de Proteínas Quinasas/metabolismo; Multimerización de Proteína/genética; Proteínas Proto-Oncogénicas B-raf/antagonistas & inhibidores; Proteínas Proto-Oncogénicas B-raf/genética; Proteínas Proto-Oncogénicas B-raf/metabolismo; Vemurafenib/metabolismo

Palabras clave

BRAF; dimerization; drug resistance; molecular dynamics; trans-autophosphorylation

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas Proto-Oncogénicas B-raf Límite: Humans Idioma: En Revista: Chembiochem Asunto de la revista: BIOQUIMICA Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos

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