Targeted delivery of antimicrobial peptide by Cry protein crystal to treat intramacrophage infection.
Biomaterials
; 217: 119286, 2019 10.
Article
en En
| MEDLINE
| ID: mdl-31284125
ABSTRACT
Antimicrobial peptides (AMPs) have recently attracted great attention due to their rapid action, broad spectrum of activity, and low propensity of resistance development. The successful application of AMPs in the treatment of intracellular infections, however, remains a challenge because of their low penetration efficiency into the pathogen's intracellular niche. Herein, we report that sub-micrometer-sized crystals of the protein Cry3Aa formed within Bacillus thuringiensis are readily and specifically taken up by macrophages. We demonstrate that these protein crystals efficiently encapsulate a known antileishmanial peptide, dermaseptin S1 (DS1), and thereby promote improved cellular uptake of DS1 and its lysosomal accumulation in macrophages. Notably, this targeted delivery of DS1 results in enhanced in vitro and in vivo antileishmanial activity, as well as reduced toxicity to the host macrophages. These findings suggest that the Cry3Aa crystal can be an effective delivery platform for AMPs to treat intramacrophage infections.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas Bacterianas
/
Sistemas de Liberación de Medicamentos
/
Macrófagos Peritoneales
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Péptidos Catiónicos Antimicrobianos
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Endotoxinas
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Proteínas Hemolisinas
Límite:
Animals
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Female
/
Humans
Idioma:
En
Revista:
Biomaterials
Año:
2019
Tipo del documento:
Article
País de afiliación:
China