Isolation, structural characterizations and bioactivities of exopolysaccharides produced by Bacillus licheniformis.
Int J Biol Macromol
; 141: 298-306, 2019 Dec 01.
Article
en En
| MEDLINE
| ID: mdl-31465808
ABSTRACT
Extraction polysaccharide from microorganism is a research hotspot. In this work, a new type of water-soluble exopolysaccharides (EPS) was isolated from Bacillus licheniformis. Firstly, response surface methodology (RSM), based on a three-level, three-factor, was used to determine optimum conditions for EPS extraction. And RSM analysis indicated optimum condition was at the temperature of 8⯰C for 10.44â¯h with ethanol at a concentration of 79.22% (v/v), the maximum yield of EPS was 3.07â¯g/mL. Secondly, EPS were seperated using DEAE-Sepharose Fast Flow column chromatography and acquired two polysaccharide fractions, BL-P1 and BL-P2. BL-P1 had larger molecular weight than BL-P2 from structural analyses, because of higher content of mannose, ribose, glucuronic acid, galactose, arabinose and fructose in BL-P2. Moreover, the characterization of BL-P1 and BL-P2 was investigated with Fourier transform infrared spectroscopy (FT-IR) and nuclear magnetic resonance (NMR) spectroscopy, the results indicated that EPS was mainly composed ofâ3)-α-d-Galp-(1â, â3,5)-α-l-Araf-(1â, â3)-ß-d-Glcp-(1â, ß-d-Glcp-(1â¯ââ¯andâ4)-ß-l-Fucp-(1â¯ââ¯4)-ß-d-Xylp-(1â¯ââ¯4)-α-l-Rhap (1â¯ââ¯3) -ß-d-Manp-(4â¯ââ¯residues. In vitro antioxidant activity assay, EPS exhibited potent quenching capacities on hydroxyl and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radicals in a dose-dependent manner. Furthermore, BL-P2 had higher activity than BL-P1 in inhibiting α-amylase and α-glucosidase, which would have potential to be applied in nutraceutical and pharmaceutical industries.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Polisacáridos Bacterianos
/
Bacillus licheniformis
Idioma:
En
Revista:
Int J Biol Macromol
Año:
2019
Tipo del documento:
Article