Lymphatic Cannulation for Lymph Sampling and Molecular Delivery.
J Immunol
; 203(8): 2339-2350, 2019 10 15.
Article
en En
| MEDLINE
| ID: mdl-31519866
ABSTRACT
Unlike the blood, the interstitial fluid and the deriving lymph are directly bathing the cellular layer of each organ. As such, composition analysis of the lymphatic fluid can provide more precise biochemical and cellular information on an organ's health and be a valuable resource for biomarker discovery. In this study, we describe a protocol for cannulation of mouse and rat lymphatic collectors that is suitable for the following the "omic" sampling of pre- and postnodal lymph, collected from different anatomical districts; the phenotyping of immune cells circulating between parenchymal organs and draining lymph nodes; injection of known amounts of molecules for quantitative immunological studies of nodal trafficking and/or clearance; and monitoring an organ's biochemical omic changes in pathological conditions. Our data indicate that probing the lymphatic fluid can provide an accurate snapshot of an organ's physiology/pathology, making it an ideal target for liquid biopsy.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Cateterismo
/
Vasos Linfáticos
/
Linfa
/
Ganglios Linfáticos
Límite:
Animals
Idioma:
En
Revista:
J Immunol
Año:
2019
Tipo del documento:
Article