Anti-infliximab antibodies: How to compare old and new data?
J Pharm Biomed Anal
; 177: 112842, 2020 Jan 05.
Article
en En
| MEDLINE
| ID: mdl-31526960
ABSTRACT
BACKGROUND:
Anti-drug-antibodies (ADA) against infliximab are frequently measured in patients receiving infliximab treatment with loss of response and undetectable infliximab concentrations. Different ADA bridging assays (1st generation, 2nd generation and ready-to-use kit) have been developed successively and were applied over the last 10 years, making comparison between ADA concentrations very challenging. A cutoff of 8⯵g/ml was established to discriminate low from high ADA concentrations using the 1st generation ADA bridging assay. The objective of this study was to enable comparison of ADA concentrations determined with the different assays that were developed over the years.METHODS:
166 serum samples were collected from patients with inflammatory bowel disease treated with infliximab. 98 samples were measured simultaneously with the 1st and 2nd generation ADA assay, 67 serum samples were measured with the 2nd generation assay and the ready-to-use kit.RESULTS:
From our ADA concentration comparison experiments, we deduced that the previously established cutoff of 8⯵g/ml with the 1st generation ELISA has a similar impact as the cutoff of 374â¯ng/ml with the 2nd generation ELISA and a cutoff of 119â¯ng/ml in the ready-to-use ELISA kit.CONCLUSION:
ADA concentrations measured with the different assays were compared and a cutoff concentration was determined for each of them to distinguish between low and high ADA concentrations. These cutoff concentrations may serve as a tool for clinicians to make treatment decisions for patients with a loss of response to infliximab and undetectable infliximab serum concentrations.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Fármacos Gastrointestinales
/
Enfermedades Inflamatorias del Intestino
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Monitoreo de Drogas
/
Infliximab
/
Anticuerpos
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
J Pharm Biomed Anal
Año:
2020
Tipo del documento:
Article