A ferrofluidic hydrophobic deep eutectic solvent for the extraction of doxycycline from urine, blood plasma and milk samples prior to its determination by high-performance liquid chromatography-ultraviolet.
J Chromatogr A
; 1613: 460695, 2020 Feb 22.
Article
en En
| MEDLINE
| ID: mdl-31740033
ABSTRACT
The ferrofluid phase was prepared according to mixing magnetic nanoparticle and the hydrophobic deep eutectic solvent as a green microextraction solvent. This new composite was applied for vortex-assisted dispersive liquid-liquid microextraction (VA-HDES-ferrofluid-DLLME) of doxycycline (DOC) residual extraction and determined through high-performance liquid chromatography-ultraviolet (HPLC-UV). The characterization of magnetic nanoparticle was investigated by XRD, TEM and FESEM. The dependency of DOC microextraction to main variables and their interaction and find optimum points were undertaken using response surface methodology with either central composite design (CCD). Thus, the optimum pH, ionic strength, ferrofluid volume and vortex time for DOC extraction are determined to be 3.0, 4%w/v, 150⯵L and 7â¯min, respectively. According to this condition, linear response is found to be greater than 10-400 ngâ¯mL-1, with a correlation coefficient of 0.983. The detection and quantification limits are 3.6 and 8.5â¯ngâ¯mL-1, while the repeatability and reproducibility as precision criteria (RSD%) are 3.74% and 4.15%, respectively. The DOC recoveries in all of the urine, blood plasma and milk samples are between 86.70 and 97.48%, with RSD% lower than 5.72%.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Plasma
/
Orina
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Técnicas de Química Analítica
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Doxiciclina
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Leche
Límite:
Animals
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Humans
Idioma:
En
Revista:
J Chromatogr A
Año:
2020
Tipo del documento:
Article
País de afiliación:
Irán