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First report and genetic diversity of porcine bufavirus in China.
Sun, Yan-Kuo; Chen, Yong-Jie; Cai, Yu; Zhu, Di-Hua; Pan, Hao-Ming; Wei, Ying-Fang; Han, Xiao-Liang; Ji, Chi-Hai; Lu, Gang; Wang, Heng; Ma, Chun-Quan; Zhang, Gui-Hong.
Afiliación
  • Sun YK; College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, China.
  • Chen YJ; College of Veterinary and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, 510642, China.
  • Cai Y; College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, China.
  • Zhu DH; College of Veterinary and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, 510642, China.
  • Pan HM; College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, China.
  • Wei YF; College of Veterinary and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, 510642, China.
  • Han XL; College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, China.
  • Ji CH; College of Veterinary and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, 510642, China.
  • Lu G; College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, China.
  • Wang H; College of Veterinary and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, 510642, China.
  • Ma CQ; College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, China.
  • Zhang GH; College of Veterinary and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, 510642, China.
Virol J ; 17(1): 2, 2020 01 06.
Article en En | MEDLINE | ID: mdl-31906997
BACKGROUND: Bufavirus is a newly discovered zoonotic virus reported in numerous mammals and humans. However, the epidemiological and genetic characteristics of porcine bufaviruses (PBuVs) in China remain unclear. METHODS: To detect PBuVs in China, 384 samples (92 fecal and 292 serum specimens) were collected from 2017 to 2018, covering six provinces in China, and were evaluated by nested PCR. Further, the positive samples from different provinces were selected to obtain the complete genome of Chinese PBuVs. RESULTS: The prevalence rate of PBuV was 16.7% in Chinese domestic pigs in the Guangdong, Guangxi, Fujian, Jiangxi, Anhui, and Henan provinces. Additionally, the positive rate of fecal specimens was higher than that of the serum samples. Next, we sequenced nine near-complete genomes of Chinese field PBuV strains from different provinces. Homology and phylogenetic analyses indicated that Chinese PBuVs have high genetic variation (93.3-99.2%), showed higher nucleotide identity with an Austrian PBuV strain (KU867071.1), and developed into different branches within the same cluster. CONCLUSION: To our knowledge, this is the first report on PBuV in China, expanding the geographic boundaries of PBuV circulation. Our data demonstrate that PBuVs are widely distributed in the six Chinese provinces. Moreover, these Chinese PBuVs exhibit genetic variation and continuous evolution characteristics. Taken together, our findings provide a foundation for future studies on bufaviruses.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Enfermedades de los Porcinos / Variación Genética / Infecciones por Parvoviridae / Parvovirinae Tipo de estudio: Prevalence_studies / Risk_factors_studies Límite: Animals País/Región como asunto: Asia Idioma: En Revista: Virol J Asunto de la revista: VIROLOGIA Año: 2020 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Enfermedades de los Porcinos / Variación Genética / Infecciones por Parvoviridae / Parvovirinae Tipo de estudio: Prevalence_studies / Risk_factors_studies Límite: Animals País/Región como asunto: Asia Idioma: En Revista: Virol J Asunto de la revista: VIROLOGIA Año: 2020 Tipo del documento: Article País de afiliación: China