A DnaJ protein that interacts with soybean mosaic virus coat protein serves as a key susceptibility factor for viral infection.
Virus Res
; 281: 197870, 2020 05.
Article
en En
| MEDLINE
| ID: mdl-31962064
ABSTRACT
Soybean mosaic virus (SMV)-disease is one of the most serious and widespread diseases in soybean (Glycine max). In the present study, a DnaJ protein in soybean designated GmCPIP (SMV coat protein-interacting protein) was screened by the QIS-Seq (quantitative interactor screening with next-generation sequencing) method, and the interaction between SMV CP and GmCPIP was confirmed by the yeast two-hybrid (Y2H) system and bimolecular fluorescence complementation (BiFC) assay. Subcellular localization analysis indicated that both proteins are localized in the cytoplasm, cytomembrane and nucleus. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis showed that infection with SMV-SC4 temporarily increased the transcription of GmCPIP. Virus-induced gene silencing (VIGS) down-regulated the GmCPIP gene by 82%, and the accumulation of SMV was decreased by 88.6% in GmCPIP-silenced plants inoculated with SMV-SC4. The interaction of GmCPIP with SMV CP seems to contribute to SMV infection in soybean.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Enfermedades de las Plantas
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Proteínas de Plantas
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Glycine max
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Virosis
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Potyvirus
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Proteínas de la Cápside
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Proteínas del Choque Térmico HSP40
Idioma:
En
Revista:
Virus Res
Asunto de la revista:
VIROLOGIA
Año:
2020
Tipo del documento:
Article
País de afiliación:
China