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Small-RNA-mediated transgenerational silencing of histone genes impairs fertility in piRNA mutants.
Barucci, Giorgia; Cornes, Eric; Singh, Meetali; Li, Blaise; Ugolini, Martino; Samolygo, Aleksei; Didier, Celine; Dingli, Florent; Loew, Damarys; Quarato, Piergiuseppe; Cecere, Germano.
Afiliación
  • Barucci G; Mechanisms of Epigenetic Inheritance, Department of Developmental and Stem Cell Biology, Institut Pasteur, UMR 3738, CNRS, Paris, France.
  • Cornes E; Sorbonne Université, Collège doctoral, Paris, France.
  • Singh M; Mechanisms of Epigenetic Inheritance, Department of Developmental and Stem Cell Biology, Institut Pasteur, UMR 3738, CNRS, Paris, France.
  • Li B; Mechanisms of Epigenetic Inheritance, Department of Developmental and Stem Cell Biology, Institut Pasteur, UMR 3738, CNRS, Paris, France.
  • Ugolini M; Bioinformatics and Biostatistics Hub, C3BI, Institut Pasteur, USR 3756, CNRS, Paris, France.
  • Samolygo A; Mechanisms of Epigenetic Inheritance, Department of Developmental and Stem Cell Biology, Institut Pasteur, UMR 3738, CNRS, Paris, France.
  • Didier C; Scuola Normale Superiore, Pisa, Italy.
  • Dingli F; Mechanisms of Epigenetic Inheritance, Department of Developmental and Stem Cell Biology, Institut Pasteur, UMR 3738, CNRS, Paris, France.
  • Loew D; Moscow Institute of Physics and Technology, Moscow, Russia.
  • Quarato P; Mechanisms of Epigenetic Inheritance, Department of Developmental and Stem Cell Biology, Institut Pasteur, UMR 3738, CNRS, Paris, France.
  • Cecere G; Centre de Recherche, Laboratoire de Spectrométrie de Masse Protéomique, Institut Curie, PSL Research University, Paris, France.
Nat Cell Biol ; 22(2): 235-245, 2020 02.
Article en En | MEDLINE | ID: mdl-32015436
ABSTRACT
PIWI-interacting RNAs (piRNAs) promote fertility in many animals. However, whether this is due to their conserved role in repressing repetitive elements (REs) remains unclear. Here, we show that the progressive loss of fertility in Caenorhabditis elegans lacking piRNAs is not caused by derepression of REs or other piRNA targets but, rather, is mediated by epigenetic silencing of all of the replicative histone genes. In the absence of piRNAs, downstream components of the piRNA pathway relocalize from germ granules and piRNA targets to histone mRNAs to synthesize antisense small RNAs (sRNAs) and induce transgenerational silencing. Removal of the downstream components of the piRNA pathway restores histone mRNA expression and fertility in piRNA mutants, and the inheritance of histone sRNAs in wild-type worms adversely affects their fertility for multiple generations. We conclude that sRNA-mediated silencing of histone genes impairs the fertility of piRNA mutants and may serve to maintain piRNAs across evolution.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Histonas / Caenorhabditis elegans / Silenciador del Gen / Proteínas de Caenorhabditis elegans / ARN Interferente Pequeño / Proteínas Argonautas Límite: Animals Idioma: En Revista: Nat Cell Biol Año: 2020 Tipo del documento: Article País de afiliación: Francia
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Histonas / Caenorhabditis elegans / Silenciador del Gen / Proteínas de Caenorhabditis elegans / ARN Interferente Pequeño / Proteínas Argonautas Límite: Animals Idioma: En Revista: Nat Cell Biol Año: 2020 Tipo del documento: Article País de afiliación: Francia