How Quantum Dots Aggregation Enhances Förster Resonant Energy Transfer.
Chemphyschem
; 21(9): 853-862, 2020 05 05.
Article
en En
| MEDLINE
| ID: mdl-32084295
ABSTRACT
As luminescent quantum dots (QDs) are known to aggregate themselves through their chemical activation by carbodiimide chemistry and their functionalization with biotin molecules, we investigate both effects on the fluorescence properties of CdTe QDs and their impact on Förster Resonant Energy Transfer (FRET) occurring with fluorescent streptavidin molecules (FA). First, the QDs fluorescence spectrum undergoes significant changes during the activation step which are explained thanks to an original analytical model based on QDs intra-aggregate screening and inter-QDs FRET. We also highlight the strong influence of biotin in solution on FRET efficiency, and define the experimental conditions maximizing the FRET. Finally, a free-QD-based system and an aggregated-QD-based system are studied in order to compare their detection threshold. The results show a minimum concentration limit of 80â
nM in FA for the former while it is equal to 5â
nM for the latter, favouring monitored aggregation in the design of QDs-based biosensors.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Transferencia Resonante de Energía de Fluorescencia
/
Puntos Cuánticos
Idioma:
En
Revista:
Chemphyschem
Asunto de la revista:
BIOFISICA
/
QUIMICA
Año:
2020
Tipo del documento:
Article
País de afiliación:
Bélgica