Ethylene negatively mediates self-incompatibility response in Brassica rapa.
Biochem Biophys Res Commun
; 525(3): 600-606, 2020 05 07.
Article
en En
| MEDLINE
| ID: mdl-32115144
ABSTRACT
Self-incompatibility (SI) is a genetic mechanism most flowering plants adopted to reject self-pollen thus avoid inbreeding. In the Brassicaceae, self-pollen recognition triggers downstream signaling pathways to reject self-pollen. However, the downstream signaling pathways are not very clear. Here we show that ethylene negatively mediates self-incompatibility response of Chinese cabbage (Brassica rapa L. ssp. Pekinensis) via PCD in papilla cells. We found that ethylene signaling genes were upregulated after cross-pollination. Treating stigmas with ethylene, or suppressing the expression of a negative regulator of ethylene signaling, CONSTITUTIVE TRIPLE RESPONSE 1 (CTR1), caused PCD in papilla cells and broke down the self-incompatibility. On the other hand, treating stigmas with ethylene inhibitors, or suppressing the expression of ethylene-responsive factors (ERFs), inhibited PCD in papilla cells and the compatible pollination. Our study identified an additional signaling pathway mediating self-incompatibility responses in the Brassicaceae and also developed a new method in overcoming self-incompatibility to improve the efficiency of inbred line propagation in agriculture practice.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Brassica rapa
/
Etilenos
/
Autoincompatibilidad en las Plantas con Flores
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
2020
Tipo del documento:
Article
País de afiliación:
China