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Establishment of Murine Model of Kidney Failure Induced by Severe Ischemia-Reperfusion Injury Useful to Evaluate Transplantation and Regenerative Therapies.
Najar-Acosta, Luis Jesus; Robles-Murillo, Ana Karina; León-Moreno, Lilia Carolina; Desentis-Desentis, Maria Fernanda; García-Espinoza, Jose Antonio; Barba-Gutiérrez, Juan Pablo; Romero-Gómez, Ana Guadalupe; Del Toro-Arreola, Alicia; Daneri-Navarro, Adrian; Topete-Camacho, Antonio; Franco-Topete, Ramon Antonio; Sánchez-Zubieta, Fernando Antonio; Zepeda-Moreno, Abraham; Rivas-Carrillo, Jorge David.
Afiliación
  • Najar-Acosta LJ; Laboratory of Tissue Engineering and Transplant, Department of Physiology, University Center for Health Sciences, University of Guadalajara, Jalisco, Mexico; cGMP Cell Processing Facility, University Center for Health Sciences, University of Guadalajara, Mexico.
  • Robles-Murillo AK; Laboratory of Tissue Engineering and Transplant, Department of Physiology, University Center for Health Sciences, University of Guadalajara, Jalisco, Mexico; cGMP Cell Processing Facility, University Center for Health Sciences, University of Guadalajara, Mexico.
  • León-Moreno LC; Laboratory of Tissue Engineering and Transplant, Department of Physiology, University Center for Health Sciences, University of Guadalajara, Jalisco, Mexico.
  • Desentis-Desentis MF; Laboratory of Tissue Engineering and Transplant, Department of Physiology, University Center for Health Sciences, University of Guadalajara, Jalisco, Mexico.
  • García-Espinoza JA; Laboratory of Tissue Engineering and Transplant, Department of Physiology, University Center for Health Sciences, University of Guadalajara, Jalisco, Mexico.
  • Barba-Gutiérrez JP; Laboratory of Tissue Engineering and Transplant, Department of Physiology, University Center for Health Sciences, University of Guadalajara, Jalisco, Mexico.
  • Romero-Gómez AG; Laboratory of Tissue Engineering and Transplant, Department of Physiology, University Center for Health Sciences, University of Guadalajara, Jalisco, Mexico.
  • Del Toro-Arreola A; Biobank of Genetic Material, Cells and Tissues for Biomedical Research, University Center for Health Sciences, University of Guadalajara, Jalisco, Mexico.
  • Daneri-Navarro A; Biobank of Genetic Material, Cells and Tissues for Biomedical Research, University Center for Health Sciences, University of Guadalajara, Jalisco, Mexico.
  • Topete-Camacho A; Biobank of Genetic Material, Cells and Tissues for Biomedical Research, University Center for Health Sciences, University of Guadalajara, Jalisco, Mexico.
  • Franco-Topete RA; Department of Microbiology and Pathology, University Center for Health Sciences, University of Guadalajara, Jalisco, Mexico.
  • Sánchez-Zubieta FA; cGMP Cell Processing Facility, University Center for Health Sciences, University of Guadalajara, Mexico; Department of Human Reproduction Clinics, University Center for Health Sciences, University of Guadalajara, Jalisco, Mexico.
  • Zepeda-Moreno A; cGMP Cell Processing Facility, University Center for Health Sciences, University of Guadalajara, Mexico; Department of Human Reproduction Clinics, University Center for Health Sciences, University of Guadalajara, Jalisco, Mexico.
  • Rivas-Carrillo JD; Laboratory of Tissue Engineering and Transplant, Department of Physiology, University Center for Health Sciences, University of Guadalajara, Jalisco, Mexico; cGMP Cell Processing Facility, University Center for Health Sciences, University of Guadalajara, Mexico. Electronic address: jorgerivas@inicia
Transplant Proc ; 52(4): 1202-1205, 2020 May.
Article en En | MEDLINE | ID: mdl-32164959
ABSTRACT

BACKGROUND:

Severe ischemia-reperfusion injury (SIRI) seems to be the key factor that can significantly affect the function of both native kidneys and renal allografts. Therefore, the development of a successful strategy is of a paramount importance in both basic and clinical research.

METHODS:

To determine the effects of SIRI on the native kidney function, a murine model was planned as follows group 1 (n = 6) mice underwent to nephrectomy plus ischemia-reperfusion injury for 30 minutes; group 2 (n = 6) mice underwent to nephrectomy without ischemia-reperfusion injury and thus served as sham controls for SIRI. The results of serum creatinine (SCr) were analyzed using Mann-Whitney U tests to calculate the significance between mean values. Survival between groups was measured by Kaplan-Meier test.

RESULTS:

To reliably achieve an elevation of SCr levels animals were exposed to a SIRI. The values of SCr increased from 0.35 (SD, 0.09) mg/dL to about 2-fold within 2 days and 3-fold within the following 5 days. Under these given conditions the mice displayed signs and histologic findings of severe kidney damage. The survival rate was about 83% of the animals within a week, and they showed no capacity of complete spontaneous self-regeneration.

CONCLUSIONS:

In this study, we aim to establish a murine model with extensive structural kidney damage and significant elevation of SCr levels, which could be used in basic and translational research of transplantation and regenerative therapies.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Daño por Reperfusión / Trasplante de Riñón / Modelos Animales de Enfermedad / Insuficiencia Renal Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Transplant Proc Año: 2020 Tipo del documento: Article País de afiliación: México
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Daño por Reperfusión / Trasplante de Riñón / Modelos Animales de Enfermedad / Insuficiencia Renal Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Transplant Proc Año: 2020 Tipo del documento: Article País de afiliación: México