Examination of vesicular stomatitis virus-induced morphology changes in individual Vero cells by QMod microscopy.
Biotechniques
; 68(6): 305-310, 2020 06.
Article
en En
| MEDLINE
| ID: mdl-32202142
ABSTRACT
Viral infection of cultured cells induces changes in the biophysical characteristics of the affected cells. Advanced microscopic cameras such as Ovizio's QMod, coupled with the appropriate software, can measure a variety of characteristics on a per-cell basis. We have employed this system to monitor the progression of vesicular stomatitis virus infection in Vero cells and to describe the cellular changes associated with advancing vesicular stomatitis virus infection. The measurements of cellular characteristics are operator-independent, and the goal is to establish a robust method to mathematically determine viral infection levels in a given sample. This will provide a means to measure viral titer in a faster and less subjective way than manual reading of plaque assays or tissue culture infectious dose 50 assays.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Virosis
/
Virus de la Estomatitis Vesicular Indiana
/
Estomatitis Vesicular
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
Biotechniques
Año:
2020
Tipo del documento:
Article
País de afiliación:
Estados Unidos