Brick plots: an intuitive platform for visualizing multiparametric immunophenotyped cell clusters.
BMC Bioinformatics
; 21(1): 145, 2020 Apr 15.
Article
en En
| MEDLINE
| ID: mdl-32293253
ABSTRACT
BACKGROUND:
The advent of mass cytometry has dramatically increased the parameter limit for immunological analysis. New approaches to analysing high parameter cytometry data have been developed to ease analysis of these complex datasets. Many of these methods assign cells into population clusters based on protein expression similarity.RESULTS:
Here we introduce an additional method, termed Brick plots, to visualize these cluster phenotypes in a simplified and intuitive manner. The Brick plot method generates a two-dimensional barcode that displays the phenotype of each cluster in relation to the entire dataset. We show that Brick plots can be used to visualize complex mass cytometry data, both from fundamental research and clinical trials, as well as flow cytometry data.CONCLUSION:
Brick plots represent a new approach to visualize complex immunological data in an intuitive manner.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Espectrometría de Masas
/
Inmunofenotipificación
Límite:
Humans
Idioma:
En
Revista:
BMC Bioinformatics
Asunto de la revista:
INFORMATICA MEDICA
Año:
2020
Tipo del documento:
Article
País de afiliación:
Nueva Zelanda