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The ribotoxin α-sarcin can cleave the sarcin/ricin loop on late 60S pre-ribosomes.
Olombrada, Miriam; Peña, Cohue; Rodríguez-Galán, Olga; Klingauf-Nerurkar, Purnima; Portugal-Calisto, Daniela; Oborská-Oplová, Michaela; Altvater, Martin; Gavilanes, José G; Martínez-Del-Pozo, Álvaro; de la Cruz, Jesús; García-Ortega, Lucía; Panse, Vikram Govind.
Afiliación
  • Olombrada M; Departamento de Bioquímica y Biología Molecular, Facultad de Química, Universidad Complutense de Madrid, Spain.
  • Peña C; Institute of Biochemistry, ETH Zürich, Otto-Stern-Weg 3, CH-8093 Zürich, Switzerland.
  • Rodríguez-Galán O; Institute of Biochemistry, ETH Zürich, Otto-Stern-Weg 3, CH-8093 Zürich, Switzerland.
  • Klingauf-Nerurkar P; Institute of Medical Microbiology, University of Zürich, Gloriastrasse 30, CH-8006 Zürich, Switzerland.
  • Portugal-Calisto D; Instituto de Biomedicina de Sevilla, Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, Seville, Spain.
  • Oborská-Oplová M; Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Seville, Spain.
  • Altvater M; Institute of Biochemistry, ETH Zürich, Otto-Stern-Weg 3, CH-8093 Zürich, Switzerland.
  • Gavilanes JG; Institute of Medical Microbiology, University of Zürich, Gloriastrasse 30, CH-8006 Zürich, Switzerland.
  • Martínez-Del-Pozo Á; Institute of Medical Microbiology, University of Zürich, Gloriastrasse 30, CH-8006 Zürich, Switzerland.
  • de la Cruz J; Institute of Biochemistry, ETH Zürich, Otto-Stern-Weg 3, CH-8093 Zürich, Switzerland.
  • García-Ortega L; Institute of Medical Microbiology, University of Zürich, Gloriastrasse 30, CH-8006 Zürich, Switzerland.
  • Panse VG; Institute of Biochemistry, ETH Zürich, Otto-Stern-Weg 3, CH-8093 Zürich, Switzerland.
Nucleic Acids Res ; 48(11): 6210-6222, 2020 06 19.
Article en En | MEDLINE | ID: mdl-32365182
ABSTRACT
The ribotoxin α-sarcin belongs to a family of ribonucleases that cleave the sarcin/ricin loop (SRL), a critical functional rRNA element within the large ribosomal subunit (60S), thereby abolishing translation. Whether α-sarcin targets the SRL only in mature 60S subunits remains unresolved. Here, we show that, in yeast, α-sarcin can cleave SRLs within late 60S pre-ribosomes containing mature 25S rRNA but not nucleolar/nuclear 60S pre-ribosomes containing 27S pre-rRNA in vivo. Conditional expression of α-sarcin is lethal, but does not impede early pre-rRNA processing, nuclear export and the cytoplasmic maturation of 60S pre-ribosomes. Thus, SRL-cleaved containing late 60S pre-ribosomes seem to escape cytoplasmic proofreading steps. Polysome analyses revealed that SRL-cleaved 60S ribosomal subunits form 80S initiation complexes, but fail to progress to the step of translation elongation. We suggest that the functional integrity of a α-sarcin cleaved SRL might be assessed only during translation.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Ricina / Saccharomyces cerevisiae / Proteínas Fúngicas / Endorribonucleasas / Subunidades Ribosómicas Grandes de Eucariotas Idioma: En Revista: Nucleic Acids Res Año: 2020 Tipo del documento: Article País de afiliación: España

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Ricina / Saccharomyces cerevisiae / Proteínas Fúngicas / Endorribonucleasas / Subunidades Ribosómicas Grandes de Eucariotas Idioma: En Revista: Nucleic Acids Res Año: 2020 Tipo del documento: Article País de afiliación: España