GoldenBac: a simple, highly efficient, and widely applicable system for construction of multi-gene expression vectors for use with the baculovirus expression vector system.

Neuhold, Jana; Radakovics, Katharina; Lehner, Anita; Weissmann, Florian; Garcia, Maria Queralt; Romero, Mari Carmen; Berrow, Nicholas S; Stolt-Bergner, Peggy

Neuhold, Jana; Radakovics, Katharina; Lehner, Anita; Weissmann, Florian; Garcia, Maria Queralt; Romero, Mari Carmen; Berrow, Nicholas S; Stolt-Bergner, Peggy.

Afiliación

Neuhold J; Protein Technologies Facility, Vienna BioCenter Core Facilities, Dr. Bohr-gasse 3, 1030, Vienna, Austria.
Radakovics K; Protein Technologies Facility, Vienna BioCenter Core Facilities, Dr. Bohr-gasse 3, 1030, Vienna, Austria.
Lehner A; Protein Technologies Facility, Vienna BioCenter Core Facilities, Dr. Bohr-gasse 3, 1030, Vienna, Austria.
Weissmann F; Research Institute of Molecular Pathology, Campus Vienna Biocenter 1, 1030, Vienna, Austria.
Garcia MQ; Protein Expression Core Facility, Institute for Research in Biomedicine (IRB) Barcelona, The Barcelona Institute of Science and Technology, C/ Baldiri Reixac 10, 08028, Barcelona, Spain.
Romero MC; Protein Expression Core Facility, Institute for Research in Biomedicine (IRB) Barcelona, The Barcelona Institute of Science and Technology, C/ Baldiri Reixac 10, 08028, Barcelona, Spain.
Berrow NS; Protein Expression Core Facility, Institute for Research in Biomedicine (IRB) Barcelona, The Barcelona Institute of Science and Technology, C/ Baldiri Reixac 10, 08028, Barcelona, Spain.
Stolt-Bergner P; Protein Technologies Facility, Vienna BioCenter Core Facilities, Dr. Bohr-gasse 3, 1030, Vienna, Austria. peggy.stolt@vbcf.ac.at.

BMC Biotechnol ; 20(1): 26, 2020 05 12.

Article en En | MEDLINE | ID: mdl-32398045

Asunto(s)

Baculoviridae/genética; Clonación Molecular/métodos; Expresión Génica; Proteínas Recombinantes/genética; Animales; Enzimas de Restricción del ADN/genética; Escherichia coli/genética; Técnicas de Inactivación de Genes; Vectores Genéticos; Proteínas de Choque Térmico; Recombinación Homóloga; Reacción en Cadena de la Polimerasa; Células Sf9

Palabras clave

Baculovirus expression; Co-expression; Golden Gate cloning; Multi-gene expression; Protein complex

Texto completo

Imprimir

XML

PubMed Links

Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas Recombinantes / Expresión Génica / Baculoviridae / Clonación Molecular Límite: Animals Idioma: En Revista: BMC Biotechnol Asunto de la revista: BIOTECNOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Austria

Texto completo

Imprimir

XML

PubMed Links

Buscar en Google