GPETAFLR, a peptide from Lupinus angustifolius L. prevents inflammation in microglial cells and confers neuroprotection in brain.
Nutr Neurosci
; 25(3): 472-484, 2022 Mar.
Article
en En
| MEDLINE
| ID: mdl-32401697
ABSTRACT
OBJECTIVES:
Neuroinflammation is a complex inflammatory process in the central nervous system (CNS) where microglia may play a critical role. GPETAFLR is a peptide isolated from Lupinus angustifolius L. protein hydrolysates with functional activity in mononuclear phagocytes. However, it is unknown whether GPETAFLR has neuroprotective effects.METHODS:
We analysed the potential anti-neuroinflammatory activity of GPETAFLR by using two different models of neuroinflammation BV-2 microglial cells and mice with high-fat diet (HFD)-induced obesity.RESULTS:
GPETAFLR hampered LPS-induced upregulation of pro-inflammatory and M1 marker genes in BV-2 cells. This effect was accompanied by an unchanged expression of anti-inflammatory IL-10 gene and by an increased expression of M2 marker genes. GPETAFLR also increased the transcriptional activity of M2 marker genes, while the microglia population remained unchanged in number and M1/M2 status in brain of mice with high-fat diet (HFD)-induced obesity. Furthermore, GPETAFLR counteracted HFD-induced downregulation of IL-10 and upregulation of pro-inflammatory markers in the mouse brain, both at gene and protein levels.DISCUSSION:
This is the first report describing that a peptide from plant origin robustly restrained the pro-inflammatory activation of microglial cells in cultures and in brain. Our data suggest that GPETAFLR might be instrumental in maintaining CNS homeostasis by inhibiting neuroinflammation.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Microglía
/
Lupinus
Límite:
Animals
Idioma:
En
Revista:
Nutr Neurosci
Asunto de la revista:
CIENCIAS DA NUTRICAO
/
NEUROLOGIA
Año:
2022
Tipo del documento:
Article
País de afiliación:
España