Your browser doesn't support javascript.
loading
Targeted Delivery of Mesenchymal Stem Cell-Derived Nanovesicles for Spinal Cord Injury Treatment.
Lee, Ju-Ro; Kyung, Jae Won; Kumar, Hemant; Kwon, Sung Pil; Song, Seuk Young; Han, In-Bo; Kim, Byung-Soo.
Afiliación
  • Lee JR; School of Chemical and Biological Engineering, Seoul National University, Seoul 08826, Korea.
  • Kyung JW; Department of Neurosurgery, CHA university, CHA Bundang Medical Center, Seongnam-si 13448, Korea.
  • Kumar H; Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research-Ahmedabad, Gujarat 382010, India.
  • Kwon SP; School of Chemical and Biological Engineering, Seoul National University, Seoul 08826, Korea.
  • Song SY; School of Chemical and Biological Engineering, Seoul National University, Seoul 08826, Korea.
  • Han IB; Department of Neurosurgery, CHA university, CHA Bundang Medical Center, Seongnam-si 13448, Korea.
  • Kim BS; School of Chemical and Biological Engineering, Seoul National University, Seoul 08826, Korea.
Int J Mol Sci ; 21(11)2020 Jun 11.
Article en En | MEDLINE | ID: mdl-32545361
ABSTRACT
Due to the safety issues and poor engraftment of mesenchymal stem cell (MSC) implantation, MSC-derived exosomes have been spotlighted as an alternative therapy for spinal cord injury (SCI). However, insufficient productivity of exosomes limits their therapeutic potential for clinical application. Moreover, low targeting ability of unmodified exosomes is a critical obstacle for their further applications as a therapeutic agent. In the present study, we fabricated macrophage membrane-fused exosome-mimetic nanovesicles (MF-NVs) from macrophage membrane-fused umbilical cord blood-derived MSCs (MF-MSCs) and confirmed their therapeutic potential in a clinically relevant mouse SCI model (controlled mechanical compression injury model). MF-NVs contained larger quantity of ischemic region-targeting molecules compared to normal MSC-derived nanovesicles (N-NVs). The targeting molecules in MF-NVs, which were derived from macrophage membranes, increased the accumulation of MF-NVs in the injured spinal cord after the in vivo systemic injection. Increased accumulation of MF-NVs attenuated apoptosis and inflammation, prevented axonal loss, enhanced blood vessel formation, decreased fibrosis, and consequently, improved spinal cord function. Synthetically, we developed targeting efficiency-potentiated exosome-mimetic nanovesicles and present their possibility of clinical application for SCI.
Asunto(s)
Palabras clave

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Traumatismos de la Médula Espinal / Exosomas / Células Madre Mesenquimatosas Límite: Animals / Female / Humans Idioma: En Revista: Int J Mol Sci Año: 2020 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Traumatismos de la Médula Espinal / Exosomas / Células Madre Mesenquimatosas Límite: Animals / Female / Humans Idioma: En Revista: Int J Mol Sci Año: 2020 Tipo del documento: Article