Single-Molecule Studies of Protein Folding with Optical Tweezers.
Annu Rev Biochem
; 89: 443-470, 2020 06 20.
Article
en En
| MEDLINE
| ID: mdl-32569525
ABSTRACT
Manipulation of individual molecules with optical tweezers provides a powerful means of interrogating the structure and folding of proteins. Mechanical force is not only a relevant quantity in cellular protein folding and function, but also a convenient parameter for biophysical folding studies. Optical tweezers offer precise control in the force range relevant for protein folding and unfolding, from which single-molecule kinetic and thermodynamic information about these processes can be extracted. In this review, we describe both physical principles and practical aspects of optical tweezers measurements and discuss recent advances in the use of this technique for the study of protein folding. In particular, we describe the characterization of folding energy landscapes at high resolution, studies of structurally complex multidomain proteins, folding in the presence of chaperones, and the ability to investigate real-time cotranslational folding of a polypeptide.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Ribosomas
/
Biosíntesis de Proteínas
/
Chaperonas Moleculares
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Proteoma
/
Escherichia coli
/
Pinzas Ópticas
Límite:
Humans
Idioma:
En
Revista:
Annu Rev Biochem
Año:
2020
Tipo del documento:
Article