Loop-mediated isothermal amplification (LAMP) reaction as viable PCR substitute for diagnostic applications: a comparative analysis study of LAMP, conventional PCR, nested PCR (nPCR) and real-time PCR (qPCR) based on Entamoeba histolytica DNA derived from faecal sample.

Foo, Phiaw Chong; Nurul Najian, A B; Muhamad, Nuramin A; Ahamad, Mariana; Mohamed, Maizan; Yean Yean, Chan; Lim, Boon Huat

Foo, Phiaw Chong; Nurul Najian, A B; Muhamad, Nuramin A; Ahamad, Mariana; Mohamed, Maizan; Yean Yean, Chan; Lim, Boon Huat.

Afiliación

Foo PC; Acarology Unit, Infectious Disease Research Centre, Institute for Medical Research, Ministry of Health Malaysia, National Institutes of Health Complex, Bandar Setia Alam, 40170, Shah Alam, Selangor, Malaysia. pcfoo12@gmail.com.
Nurul Najian AB; Department of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia, Health Campus, 16150, Kubang Kerian, Kelantan, Malaysia. pcfoo12@gmail.com.
Muhamad NA; Department of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia, Health Campus, 16150, Kubang Kerian, Kelantan, Malaysia.
Ahamad M; Bacteriology Unit, Infectious Disease Research Centre, Institute for Medical Research, Ministry of Health Malaysia, National Institutes of Health Complex, Bandar Setia Alam, 40170, Shah Alam, Selangor, Malaysia.
Mohamed M; Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Health Campus, 16150, Kubang Kerian, Kelantan, Malaysia.
Yean Yean C; Acarology Unit, Infectious Disease Research Centre, Institute for Medical Research, Ministry of Health Malaysia, National Institutes of Health Complex, Bandar Setia Alam, 40170, Shah Alam, Selangor, Malaysia.
Lim BH; Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, City Campus, Pengkalan Chepa, Locked bag 36, 16100, Kota Bharu, Kelantan, Malaysia.

BMC Biotechnol ; 20(1): 34, 2020 06 22.

Article en En | MEDLINE | ID: mdl-32571286

Asunto(s)

Entamoeba histolytica/genética; Entamoeba histolytica/aislamiento & purificación; Entamebiasis/diagnóstico; Técnicas de Diagnóstico Molecular/métodos; Técnicas de Amplificación de Ácido Nucleico/métodos; Reacción en Cadena en Tiempo Real de la Polimerasa/métodos; Anticuerpos Antiprotozoarios/inmunología; Antígenos de Protozoos; Cartilla de ADN/genética; ADN Protozoario/genética; Entamoeba/clasificación; Entamoeba/genética; Entamoeba/aislamiento & purificación; Entamebiasis/microbiología; Heces/parasitología; Inmunoensayo; Límite de Detección; Reacción en Cadena de la Polimerasa/métodos; Sensibilidad y Especificidad

Palabras clave

Calcein-manganese visualization; LAMP analytical sensitivity; Lateral flow dipstick; Loop-mediated isothermal amplification; Nested PCR; Real-time PCR

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Técnicas de Amplificación de Ácido Nucleico / Técnicas de Diagnóstico Molecular / Entamoeba histolytica / Entamebiasis / Reacción en Cadena en Tiempo Real de la Polimerasa Tipo de estudio: Diagnostic_studies / Prognostic_studies Idioma: En Revista: BMC Biotechnol Asunto de la revista: BIOTECNOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Malasia

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