Acquired pellicle protein-based engineering protects against erosive demineralization.
J Dent
; 102: 103478, 2020 11.
Article
en En
| MEDLINE
| ID: mdl-32950632
ABSTRACT
OBJECTIVES:
To evaluate, in vivo 1) proteomic alterations in the acquired enamel pellicle (AEP) after treatment with sugarcane-derived cystatin (CaneCPI-5), hemoglobin (HB), statherin-derived peptide (StN15) or their combination before the formation of the AEP and subsequent erosive challenge; 2) the protection of these treatments against erosive demnineralization. MATERIALS ANDMETHODS:
In 5 crossover phases, after prophylaxis, 10 volunteers rinsed (10â¯mL, 1â¯min) with deionized water-1, 0.1â¯mg/mL CaneCPI-5-2, 1.0â¯mg/mL HB-3, 1.88â¯×â¯10-5 M StN15-4 or their combination-5. AEP was formed (2â¯h) and enamel biopsy (10⯵L, 1%citric acid, pH 2.5, 10â¯s) was performed on one incisor for calcium analysis. The same acid was applied on the vestibular surfaces of the remaining teeth. The acid-resistant proteins within the remaining AEP were collected. Samples were quantitatively analyzed by label-free proteomics.RESULTS:
Treatment with the proteins/peptide, isolated or combined, increased several acid-resistant proteins in the AEP, compared with control. The highest increases were seen for PRPs (32-fold, StN15), profilin (15-fold, combination), alpha-amylase (9-fold; StN15), keratins (8-fold, CaneCPI-5 and HB), Histatin-1 (7-fold, StN15), immunoglobulins (6.5-fold, StN15), lactotransferrin (4-fold, CaneCPI-5), cystatins, lysozyme, protein S-100-A9 and actins (3.5-fold, StN15), serum albumin (3.5-fold, CaneCPI-5 and HB) and hemoglobin (3-fold, StN15). Annexin, calmodulin, keratin, tubulin and cystatins were identified exclusively upon treatment with the proteins/peptide, alone or combined. Groups 2, 3 and 4 had significantly lower Ca released from enamel compared to group 1 (Kruskal-Wallis/Dunn's, pâ¯<â¯0.05).CONCLUSIONS:
Treatment with CaneCPI-5, HB or StN15 remarkably increases acid-resistant proteins in the AEP, protecting against erosion. CLINICALSIGNIFICANCE:
Our results show, for the first time, that treatment with proteins/peptide remarkably increases acid-resistant proteins in the AEP, protecting against erosive demineralization. These findings open an avenue for a new preventive approach for erosive demineralization, employing acquired pellicle engineering procedures that may in the future be incorporated into dental products.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Erosión de los Dientes
/
Desmineralización Dental
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
J Dent
Año:
2020
Tipo del documento:
Article
País de afiliación:
Brasil