Maternal undernutrition modulates hepatic MicroRNAs expression in the early life of offspring.
Exp Cell Res
; 400(2): 112450, 2021 03 15.
Article
en En
| MEDLINE
| ID: mdl-33347859
ABSTRACT
Emerging studies revealed that a poor intrauterine environment elicited by maternal nutrient restriction (MNR) is associated with an increased risk of metabolic diseases in adulthood. Previous research has shown that microRNAs (miRNAs) exert pivotal roles in modulating molecular pathways involved in disease pathogenesis and progression. In this respect, we herein examined miRNA profiles in samples of liver from offspring whose mothers were fed either with a 50% food-restricted diet or standard laboratory chow during pregnancy. Our findings enumerated that miR-181a, involved in lipid metabolism, was found to be downregulated in the liver of MNR offspring at 1 day of age when compared to that of control offspring. We also noted that overexpression of miR-181a reduced the lipid droplets after treatment with oleic acid for 48 h, which suppressed the expressions levels of SIRT1, FOXO1, KLF6 and PPARγ in BRL-3A cells, while the opposite results were observed with decreased expression of miR-181a. Furthermore, the luciferase reporter assay confirmed the direct interactions between miR-181a with KLF6 and SIRT1. In adults, the MNR offspring elucidated increased TG content, decreased expression of miR-181a, and increased expressions levels of SIRT1, FOXO1, KLF6, and PPARγ in liver tissues. Collectively, these findings provided novel evidence that MNR could regulate miRNAs expression, which might be related to lipid metabolism in MNR offspring.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Efectos Tardíos de la Exposición Prenatal
/
MicroARNs
/
Fenómenos Fisiologicos Nutricionales Maternos
/
Desnutrición
/
Hígado
/
Intercambio Materno-Fetal
Tipo de estudio:
Etiology_studies
Límite:
Animals
/
Pregnancy
Idioma:
En
Revista:
Exp Cell Res
Año:
2021
Tipo del documento:
Article
País de afiliación:
China