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A Mouse Model to Investigate the Role of Cancer-associated Fibroblasts in Tumor Growth.
Jelinek, David; Zhang, Ellen Ran; Ambrus, Aaron; Haley, Erin; Guinn, Emily; Vo, Austin; Le, Peter; Kesaf, Ayse Elif; Nguyen, Jennifer; Guo, Lily; Frederick, Destiny; Sun, Zhengyang; Guo, Natalie; Sevier, Parker; Bilotta, Eric; Atai, Kaiser; Voisin, Laurent; Coller, Hilary A.
Afiliación
  • Jelinek D; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles; Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles.
  • Zhang ER; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles; Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles; Department of Molecular Biology, Princeton University.
  • Ambrus A; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles; Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles.
  • Haley E; Department of Molecular Biology, Princeton University.
  • Guinn E; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles; Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles.
  • Vo A; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles; Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles.
  • Le P; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles; Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles.
  • Kesaf AE; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles; Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles.
  • Nguyen J; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles; Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles.
  • Guo L; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles; Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles.
  • Frederick D; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles; Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles.
  • Sun Z; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles; Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles.
  • Guo N; Department of Molecular Biology, Princeton University.
  • Sevier P; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles; Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles.
  • Bilotta E; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles; Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles.
  • Atai K; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles; Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles; Molecular Biology Institute, University of California, Los Angeles.
  • Voisin L; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles; Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles.
  • Coller HA; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles; Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles; Molecular Biology Institute, University of California, Los Angeles; hcoller@ucla.edu.
J Vis Exp ; (166)2020 12 22.
Article en En | MEDLINE | ID: mdl-33427239
Cancer-associated fibroblasts (CAFs) can play an important role in tumor growth by creating a tumor-promoting microenvironment. Models to study the role of CAFs in the tumor microenvironment can be helpful for understanding the functional importance of fibroblasts, fibroblasts from different tissues, and specific genetic factors in fibroblasts. Mouse models are essential for understanding the contributors to tumor growth and progression in an in vivo context. Here, a protocol in which cancer cells are mixed with fibroblasts and introduced into mice to develop tumors is provided. Tumor sizes over time and final tumor weights are determined and compared among groups. The protocol described can provide more insight into the functional role of CAFs in tumor growth and progression.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Fibroblastos Asociados al Cáncer / Neoplasias Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Animals / Humans Idioma: En Revista: J Vis Exp Año: 2020 Tipo del documento: Article
Texto completo
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Fibroblastos Asociados al Cáncer / Neoplasias Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Animals / Humans Idioma: En Revista: J Vis Exp Año: 2020 Tipo del documento: Article