Monoclonal antibodies to bovine serum albumin: affinity and specificity determinations.
Mol Immunol
; 25(1): 7-15, 1988 Jan.
Article
en En
| MEDLINE
| ID: mdl-3343974
ABSTRACT
A panel of 12 monoclonal antibodies (MAb) to bovine serum albumin (BSA) was developed and characterized as to their physiochemical and immunological properties. Affinity constants of the MAb varied over a wide range from 10(5) to 10(8) M-1. MAb were assembled into several groups of non- or minimally interacting antibodies by analysis of competitive binding experiments, and BSA domain and subdomain specificities of the MAb were assigned by analysis of results of MAb binding to purified BSA fragments. Further fine specificity delineation was accomplished by examination of cross-reactivity patterns to several mammalian albumins. The data suggest that some of the low affinity MAb recognize sites on different portions of the BSA molecule, indicating that similar epitopes exist on different domains of the BSA molecule.
Buscar en Google
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Albúmina Sérica Bovina
/
Anticuerpos Monoclonales
/
Afinidad de Anticuerpos
/
Especificidad de Anticuerpos
Límite:
Animals
Idioma:
En
Revista:
Mol Immunol
Año:
1988
Tipo del documento:
Article