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Epithelial cell adhesion molecule promotes breast cancer resistance protein-mediated multidrug resistance in breast cancer by inducing partial epithelial-mesenchymal transition.
Shi, Rui-Zan; He, Yi-Fan; Wen, Jie; Niu, Ya-Nan; Gao, Yu; Liu, Lin-Hong; Zhang, Xuan-Ping; Wang, Yan; Zhang, Xiu-Li; Zhang, Hui-Feng; Chen, Min; Hu, Xiao-Ling.
Afiliación
  • Shi RZ; Department of Pharmacology, Shanxi Medical University, Taiyuan, Shanxi, China.
  • He YF; Department of Pharmacology, Shanxi Medical University, Taiyuan, Shanxi, China.
  • Wen J; Department of Pharmacology, Shanxi Medical University, Taiyuan, Shanxi, China.
  • Niu YN; Department of Pharmacology, Shanxi Medical University, Taiyuan, Shanxi, China.
  • Gao Y; Department of Pharmacology, Shanxi Medical University, Taiyuan, Shanxi, China.
  • Liu LH; Department of Pharmacology, Shanxi Medical University, Taiyuan, Shanxi, China.
  • Zhang XP; Department of Pharmacology, Shanxi Medical University, Taiyuan, Shanxi, China.
  • Wang Y; Department of Pharmacology, Shanxi Medical University, Taiyuan, Shanxi, China.
  • Zhang XL; Technology Innovation Center, Hunan University of Chinese Medicine, Changsha, Hunan, China.
  • Zhang HF; Department of Pharmacology, Shanxi Medical University, Taiyuan, Shanxi, China.
  • Chen M; Department of Pharmacology, Shanxi Medical University, Taiyuan, Shanxi, China.
  • Hu XL; Department of Pharmacology, Shanxi Medical University, Taiyuan, Shanxi, China.
Cell Biol Int ; 45(8): 1644-1653, 2021 Aug.
Article en En | MEDLINE | ID: mdl-33760350
Overexpression of breast cancer resistance protein (BCRP) plays a crucial role in the acquired multidrug resistance (MDR) in breast cancer. The elucidation of molecular events that confer BCRP-mediated MDR is of major therapeutic importance in breast cancer. Epithelial cell adhesion molecule (EpCAM) has been implicated in tumor progression and drug resistance in various types of cancers, including breast cancer. However, the role of EpCAM in BCRP-mediated MDR in breast cancer remains unknown. In the present study, we revealed that EpCAM expression was upregulated in BCRP-overexpressing breast cancer MCF-7/MX cells, and EpCAM knockdown using siRNA reduced BCRP expression and increased the sensitivity of MCF-7/MX cells to mitoxantrone (MX). The epithelial-mesenchymal transition (EMT) promoted BCRP-mediated MDR in breast cancer cells, and EpCAM knockdown partially suppressed EMT progression in MCF-7/MX cells. In addition, Wnt/ß-catenin signaling was activated in MCF-7/MX cells, and the inhibition of this signaling attenuated EpCAM and BCRP expression and partially reversed EMT. Together, this study illustrates that EpCAM upregulation by Wnt/ß-catenin signaling induces partial EMT to promote BCRP-mediated MDR resistance in breast cancer cells. EpCAM may be a potential therapeutic target for overcoming BCRP-mediated resistance in human breast cancer.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Neoplasias de la Mama / Resistencia a Múltiples Medicamentos / Resistencia a Antineoplásicos / Transición Epitelial-Mesenquimal / Transportador de Casetes de Unión a ATP, Subfamilia G, Miembro 2 / Molécula de Adhesión Celular Epitelial / Proteínas de Neoplasias Límite: Female / Humans Idioma: En Revista: Cell Biol Int Año: 2021 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Neoplasias de la Mama / Resistencia a Múltiples Medicamentos / Resistencia a Antineoplásicos / Transición Epitelial-Mesenquimal / Transportador de Casetes de Unión a ATP, Subfamilia G, Miembro 2 / Molécula de Adhesión Celular Epitelial / Proteínas de Neoplasias Límite: Female / Humans Idioma: En Revista: Cell Biol Int Año: 2021 Tipo del documento: Article País de afiliación: China