Development of Antigen-specific Chimeric Antigen Receptor KHYG-1 Cells for Glioblastoma.
Anticancer Res
; 41(4): 1811-1819, 2021 Apr.
Article
en En
| MEDLINE
| ID: mdl-33813386
ABSTRACT
BACKGROUND/AIM:
Glioblastoma is the most common cancer among primary brain tumors, however, its prognosis and treatment advances are very poor. Here, we investigated whether c-Met, FOLR1, and AXL proteins are promising targets for chimeric antigen receptor (CAR) T-cell therapy, for they are known to be over-expressed in a variety of solid tumors. MATERIALS ANDMETHODS:
CAR constructs were prepared and CAR KHYG-1 cells targeting c-Met, FOLR1, or AXL were made by lentiviral transduction. The activity of CAR KHYG-1 cells against cancer cells was measured by cytokine secretion and cell lysis assays.RESULTS:
c-Met and AXL were over-expressed in most glioblastoma cell lines (11/13), but not in neuroblastoma cell lines (0/8). FOLR1 was over-expressed only in one among 16 glioblastoma cell lines. Our antigen-specific CAR KHYG-1 cells eradicated target positive glioblastoma cells selectively.CONCLUSION:
Anti-c-Met and anti-AXL CAR NK or T cells could be effective in glioblastoma cells.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Neoplasias Encefálicas
/
Células Asesinas Naturales
/
Linfocitos T
/
Inmunoterapia Adoptiva
/
Proteínas Proto-Oncogénicas
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Proteínas Tirosina Quinasas Receptoras
/
Glioblastoma
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Proteínas Proto-Oncogénicas c-met
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Receptores Quiméricos de Antígenos
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Anticancer Res
Año:
2021
Tipo del documento:
Article