COPII collar defines the boundary between ER and ER exit site and does not coat cargo containers.
J Cell Biol
; 220(6)2021 06 07.
Article
en En
| MEDLINE
| ID: mdl-33852719
ABSTRACT
COPII and COPI mediate the formation of membrane vesicles translocating in opposite directions within the secretory pathway. Live-cell and electron microscopy revealed a novel mode of function for COPII during cargo export from the ER. COPII is recruited to membranes defining the boundary between the ER and ER exit sites, facilitating selective cargo concentration. Using direct observation of living cells, we monitored cargo selection processes, accumulation, and fission of COPII-free ERES membranes. CRISPR/Cas12a tagging, the RUSH system, and pharmaceutical and genetic perturbations of ER-Golgi transport demonstrated that the COPII coat remains bound to the ER-ERES boundary during protein export. Manipulation of the cargo-binding domain in COPII Sec24B prohibits cargo accumulation in ERES. These findings suggest a role for COPII in selecting and concentrating exported cargo rather than coating Golgi-bound carriers. These findings transform our understanding of coat proteins' role in ER-to-Golgi transport.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Vesículas Cubiertas por Proteínas de Revestimiento
/
Proteínas de Transporte Vesicular
/
Retículo Endoplásmico
/
Aparato de Golgi
Límite:
Humans
Idioma:
En
Revista:
J Cell Biol
Año:
2021
Tipo del documento:
Article
País de afiliación:
Israel