Discrimination of α-Thrombin and γ-Thrombin Using Aptamer-Functionalized Nanopore Sensing.
Anal Chem
; 93(22): 7889-7897, 2021 06 08.
Article
en En
| MEDLINE
| ID: mdl-34038092
ABSTRACT
Protein detection and identification at the single-molecule level are major challenges in many biotechnological fields. Solid-state nanopores have raised attention as label-free biosensors with high sensitivity. Here, we use solid-state nanopore sensing to discriminate two closely related proteins, α-thrombin and γ-thrombin. We show that aptamer functionalization improves protein discrimination thanks to a significant difference in the relative current blockade amplitude. To enhance discrimination, we postprocessed the signals using machine learning and training algorithms and we were able to reach an accuracy of 98.8% using seven features and ensemble methods.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Técnicas Biosensibles
/
Nanoporos
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Anal Chem
Año:
2021
Tipo del documento:
Article
País de afiliación:
Francia