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Real-time imaging of viscosity in the mitochondrial matrix by a red-emissive molecular rotor.
Yuan, Lin; Wang, Dong; Shan, Shan; Chen, Juan; Huang, Wei; Han, Guangmei; Tian, Xiaohe; Zhang, Ruilong; Zhang, Zhongping; Liu, Zhengjie.
Afiliación
  • Yuan L; School of Chemistry and Chemical Engineering, Institute of Physical Science and Information Technology, Anhui University, Hefei 230601, China. zjliu@ahu.edu.cn.
Anal Methods ; 13(28): 3181-3186, 2021 07 28.
Article en En | MEDLINE | ID: mdl-34169932
ABSTRACT
Mitochondrial matrix contains numerous metabolism-related proteins/enzymes and nucleic acids, which play key roles in the process of energy generation and signal transduction. The fluctuations in mitochondrial biomacromolecular levels lead to the changes in the mitochondrial matrix viscosity; therefore, real-time measuring the mitochondrial matrix viscosity is of great significance for the in-depth understanding of the mitochondrial physiology and pathobiology. However, investigations are limited due to the lack of a mitochondrial matrix-specific molecular rotor. Herein, we report a design of a molecular rotor that is specifically enriched in the mitochondrial matrix. The red fluorescence of the rotor switches on when the viscosity increases, enabling the real-time monitoring of the viscosity change therein. Interestingly, the rotor showed non-fluorescence behaviour in the liposome (mimicking membrane structure), avoiding fluorescence interference from the mitochondrial bilayer membrane. Super-resolution imaging reveals that the viscosity is uneven in an individual mitochondrion.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Colorantes Fluorescentes / Mitocondrias Tipo de estudio: Diagnostic_studies Idioma: En Revista: Anal Methods Año: 2021 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Colorantes Fluorescentes / Mitocondrias Tipo de estudio: Diagnostic_studies Idioma: En Revista: Anal Methods Año: 2021 Tipo del documento: Article País de afiliación: China