Real-time imaging of viscosity in the mitochondrial matrix by a red-emissive molecular rotor.
Anal Methods
; 13(28): 3181-3186, 2021 07 28.
Article
en En
| MEDLINE
| ID: mdl-34169932
ABSTRACT
Mitochondrial matrix contains numerous metabolism-related proteins/enzymes and nucleic acids, which play key roles in the process of energy generation and signal transduction. The fluctuations in mitochondrial biomacromolecular levels lead to the changes in the mitochondrial matrix viscosity; therefore, real-time measuring the mitochondrial matrix viscosity is of great significance for the in-depth understanding of the mitochondrial physiology and pathobiology. However, investigations are limited due to the lack of a mitochondrial matrix-specific molecular rotor. Herein, we report a design of a molecular rotor that is specifically enriched in the mitochondrial matrix. The red fluorescence of the rotor switches on when the viscosity increases, enabling the real-time monitoring of the viscosity change therein. Interestingly, the rotor showed non-fluorescence behaviour in the liposome (mimicking membrane structure), avoiding fluorescence interference from the mitochondrial bilayer membrane. Super-resolution imaging reveals that the viscosity is uneven in an individual mitochondrion.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Colorantes Fluorescentes
/
Mitocondrias
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Anal Methods
Año:
2021
Tipo del documento:
Article
País de afiliación:
China